Proteomic Study of the Exponential-Stationary Growth Phase Transition in the Haloarchaea Natrialba magadii and Haloferax volcanii

GIMÉNEZ, MARÍA INES; POETSCH, ANSGAR; TRÖETSCHEL, CHRISTIAN; DE CASTRO, ROSANA ESTER; CERLETTI, MICAELA; D'ALESSANDRO, CELESTE; PAGGI, ROBERTO A.

PROTEOMICS (WEINHEIM. PRINT)

WILEY-V C H VERLAG GMBH

Lugar: Weinheim; Año: 2018 vol. 18

1615-9853

The dynamic changes that take place along the phases of microbial growth(lag, exponential, stationary, and death) have been widely studied in bacteriaat the molecular and cellular levels, but little is known for archaea. In thisstudy, a high-throughput approach was used to analyze and compare theproteomes of two haloarchaea during exponential and stationary growth: theneutrophilic Haloferax volcanii and the alkaliphilic Natrialba magadii. Almost2000 proteins were identified in each species (50% of the predictedproteome). Among them, 532 and 432 were found to be differential betweengrowth phases in H. volcanii and N. magadii, respectively. Changes uponentrance into stationary phase included an overall increase in proteinsinvolved in the transport of small molecules and ions, stress response, andfatty acid catabolism. Proteins related to genetic processes and cell divisionshowed a notorious decrease in amount. The data reported in this study notonly contributes to our understanding of the exponential?stationary growthphase transition in extremophilic archaea but also provides the firstcomprehensive analysis of the proteome composition of N. magadii. TheMSproteomics data have been deposited in the ProteomeXchange Consortiumwith the dataset identifier JPST000395.