The sesquiterpene botrydial from Botrytis cinerea induces phosphatidic acid production in tomato cell suspensions

D'AMBROSIO, JUAN MARTIN; DI PALMA, ANDRÉS ARRUEBARRENA; LAXALT, ANA MARIA; SUELDO, DANIELA J.; LAMATTINA, LORENZO; GONORAZKY, GABRIELA; MORAGA, JAVIER; COLLADO, ISIDRO GONZÁLEZ

PLANTA

SPRINGER

Lugar: Berlin; Año: 2018 vol. 247 p. 1001 - 1009

0032-0935

Phosphatidic acid (PA) is a phospholipid second messenger involved in the induction of plant defense responses. It is generated via two distinct enzymatic pathways, either via phospholipase D (PLD) or by the sequential action of phospholipase C and diacylglycerol kinase (PLC/DGK). Botrydial is a phytotoxic sesquiterpene generated by the necrotrophic fungus Botrytis cinerea that induces diverse plant defense responses, such as the production of reactive oxygen species (ROS). Here we analyzed PA and ROS production and their interplay upon botrydial treatments, employing tomato cell suspensions as a model system. We observed that botrydial induces PA production within minutes via PLD and PLC/DGK. We also found that either inhibition of PLC or DGK diminishes ROS generation triggered by botrydial. This indicates that PLC/DGK is upstream of ROS production. In tomato (Solanum lycopersicum), PLC is encoded by a multigene family constituted by SlPLC1 ? SlPLC6 and the pseudogene SlPLC7. We have shown that SlPLC2-silenced plants have reduced susceptibility to B. cinerea. In this work we studied the role of SlPLC2 on botrydial-induced PA production by silencing the expression of SlPLC2 via a specific artificial microRNA. Upon botrydial treatments, SlPLC2-silenced cell suspensions produce PA levels similar to wild type cells. It can be concluded that PA is a novel component of the plant responses triggered by botrydial