IIB   20738
INSTITUTO DE INVESTIGACIONES BIOLOGICAS
Unidad Ejecutora - UE
artículos
Título:
The NOS-like protein from the microalgae Ostreococcus tauri is a genuine and ultrafast NO-producing enzyme
Autor/es:
LORENZO LAMATTINA; MEHDI LEMBROUK; ANNIE FRELET-BARRAND; FRANÇOIS ANDRÉ; NOELIA FORESI; MARINE WEISSLOCKER-SCHAETZEL; JÉRÔME SANTOLINI; PIERRE DORLET; NABILA TOUAZI
Revista:
PLANT SCIENCE
Editorial:
ELSEVIER IRELAND LTD
Referencias:
Lugar: Amsterdam; Año: 2017 p. 100 - 111
ISSN:
0168-9452
Resumen:
The exponential increase of genomes? sequencing has revealed the presence of NO-Synthases (NOS) throughoutthe tree of life, uncovering an extraordinary diversity of genetic structure and biological functions. Although NOhas been shown to be a crucial mediator in plant physiology, NOS sequences seem present solely in green algaegenomes, with a first identification in the picoplankton species Ostreococcus tauri. There is no rationale so far toaccount for the presence of NOS in this early-diverging branch of the green lineage and its absence in land plants.To address the biological function of algae NOS, we cloned, expressed and characterized the NOS oxygenasedomain from Ostreococcus tauri (OtNOSoxy). We launched a phylogenetic and structural analysis of algae NOS,and achieved a 3D model of OtNOSoxy by homology modeling. We used a combination of various spectroscopiesto characterize the structural and electronic fingerprints of some OtNOSoxy reaction intermediates. The analysisof OtNOSoxy catalytic activity and kinetic efficiency was achieved by stoichiometric stopped-flow. Our resultshighlight the conserved and particular features of OtNOSoxy structure that might explain its ultrafast NO-producingcapacity. This integrative Structure-Catalysis-Function approach could be extended to the whole NOSsuperfamily and used for predicting potential biological activity for any new NOS.