IIB   20738
INSTITUTO DE INVESTIGACIONES BIOLOGICAS
Unidad Ejecutora - UE
artículos
Título:
Identification of tomato phosphatidylinositol-specific phospholipase-C (PI-PLC) family members and the role of PLC4 and PLC6 in HR and disease resistance
Autor/es:
JACK H. VOSSEN1,†,‡, AHMED ABD-EL-HALIEM1,†, EMILIE F. FRADIN1, GRARDY C.M. VAN DEN BERG1, SOPHIA K. EKENGREN2,§, HAROLD J.G. MEIJER1, ALIREZA SEIFI3, YULING BAI3, ARJEN TEN HAVE4, TEUN MUNNIK5, BART P.H.J. THOMMA1 AND MATTHIEU H.A.J. JOOSTEN1,*
Revista:
PLANT JOURNAL
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Año: 2010 vol. 61
ISSN:
0960-7412
Resumen:
The perception of pathogen-derived elicitors by plants has been suggested to involve phosphatidylinositolspecificphospholipase-C (PI-PLC) signalling. Here we show that PLC isoforms are required for thehypersensitive response (HR) and disease resistance. We characterised the tomato [Solanum lycopersicum(Sl)] PLC gene family. Six Sl PLC-encoding cDNAs were isolated and their expression in response to infectionwith the pathogenic fungus Cladosporium fulvum was studied. We found significant regulation at thetranscriptional level of the various SlPLCs, and SlPLC4 and SlPLC6 showed distinct expression patterns inC. fulvum-resistant Cf-4 tomato. We produced the encoded proteins in Escherichia coli and found that bothgenes encode catalytically active PI-PLCs. To test the requirement of these Sl PLCs for full Cf-4-mediatedrecognition of the effector Avr4, we knocked down the expression of the encoding genes by virus-induced genesilencing. Silencing of SlPLC4 impaired the Avr4/Cf-4-induced HR and resulted in increased colonisation of Cf-4plants by C. fulvum expressing Avr4. Furthermore, expression of the gene in Nicotiana benthamiana enhancedthe Avr4/Cf-4-induced HR. Silencing of SlPLC6 did not affect HR, whereas it caused increased colonisationof Cf-4 plants by the fungus. Interestingly, Sl PLC6, but not Sl PLC4, was also required for resistance toVerticillium dahliae, mediated by the transmembrane Ve1 resistance protein, and to Pseudomonas syringae,mediated by the intracellular Pto/Prf resistance protein couple. We conclude that there is a differentialrequirement of PLC isoforms for the plant immune response and that Sl PLC4 is specifically required for Cf-4function, while Sl PLC6 may be a more general component of resistance protein signalling.