IIB 20738
INSTITUTO DE INVESTIGACIONES BIOLOGICAS
Unidad Ejecutora - UE
artículos
Título:
Extracellular ATP, nitric oxide and superoxide act coodinately to regulate hypocotyl growth in etiolated Arabidopsis seedlings
Autor/es:
TONÓN, C.; TERRILE, C.; IGLESIAS, M. J.; LAMATTINA, L.; CASALONGUE, C.
Revista:
Journal of Plant Physiology
Editorial:
Elsevier
Referencias:
Año: 2009 vol. 167 p. 540 - 546
ISSN:
0176-1617
Resumen:
Etiolated Arabidopsisthaliana seedlingsgerminatedinthepresenceofreducingbufferssuchasreduced gluthathione(GSH)anddithiothreitol(DTT)havealteredmorphology.GSHandDTTinhibitedhypocotyl elongationinadose-dependentmanner.TheGSH-mediatedeffectwaspreventedbythesimultaneous additionofextracellularATP(eATP).NADPHoxidase(NOX)activityandendogenousnitricoxide(NO) generationwererequiredtomediateeATPactiononthehypocotylelongation.Acorrelationwas observedbetweenhypocotyllength,eATPconcentrationandNOproduction.TheactionofeATPandNO on superoxide(O2d) accumulationandperoxidaseactivitywasinvestigated.TheO2d distributionwas regulatedbyeATPandNOduringhypocotylelongation.Ourdatasuggestthatafinelytunedbalance of redoxstatusandoptimallevelsofATPandNOareessentialtoregulatethehypocotylelongationin the dark.
