IIB   20738
INSTITUTO DE INVESTIGACIONES BIOLOGICAS
Unidad Ejecutora - UE
artículos
Título:
Phospholipase D δ knock-out mutants are tolerant to severe drought stress.
Autor/es:
DISTÉFANO, A.; VALIÑAS MA; SCUFFI D; LAMATTINA L; TEN HAVE A; GARCIA-MATA, C.; LAXALT AM
Revista:
Plant Signaling & Behavior
Editorial:
Taylor
Referencias:
Año: 2015 vol. 4
Resumen:
Phospholipase D (PLD) is involved in different plant processes, ranging from responses to abiotic and biotic stress toplant development. Phospholipase Dd (PLDd) is activated in dehydration and salt stress, producing the lipid secondmessenger phosphatidic acid. In this work we show that pldd Arabidopsis mutants were more tolerant to severedrought than wild-type plants. PLDd has been shown to be required for ABA regulation of stomatal closure of isolatedepidermal peels. However, there was no significant difference in stomatal conductance at the whole plant levelbetween wild-type and pldd mutants. Since PLD hydrolyses structural phospholipids, then we looked at membraneintegrity. Ion leakage measurements showed that during dehydration of leaf discs pldd mutant has less membranedegradation compared to the wild-type. We further analyzed the mutants and showed that pldd have higher mRNAlevels of RAB18 and RD29A compared to wild-type plants under normal growth conditions. Transient expression ofAtPLDd in Nicotiana benthamiana plants induced a wilting phenotype. These findings suggest that, in wt plants PLDddisrupt membranes in severe drought stress and, in the absence of the protein (PLDd knock-out) might drought-primethe plants, making them more tolerant to severe drought stress. The results are discussed in relation to PLDd role inguard cell signaling and drought tolerance.