IIB   20738
INSTITUTO DE INVESTIGACIONES BIOLOGICAS
Unidad Ejecutora - UE
artículos
Título:
Aerobic Nitric Oxide Production by Azospirillum brasilense Sp245 and its Influence on Root Architecture in Tomato
Autor/es:
MOLINA-FAVERO, C.; CREUS, C.; SIMONTACHI, M.; PANTARULO, S.; LORENZO LAMATTINA
Revista:
Molecular Plant-Microbe Interactions
Editorial:
The American Phytopathological Soc (APS)
Referencias:
Año: 2008 vol. 21 p. 1001 - 1009
ISSN:
0894-0282
Resumen:
The major feature of the plant-growth-promoting bacteria
Azospirillum brasilense is its ability to modify plant root
architecture. In plants, nitric oxide (NO) mediates indole-
3-acetic acid (IAA)-signaling pathways leading to both lateral
(LR) and adventitious (AR) root formation. Here, we
analyzed aerobic NO production by A. brasilense Sp245
wild type (wt) and its mutants Faj009 (IAA-attenuated)
and Faj164 (periplasmic nitrate reductase negative), and
its correlation with tomato root-growth-promoting effects.
The wt and Faj009 strains produced 120 nmol NO per
gram of bacteria in aerated nitrate-containing medium. In
contrast, Faj164 produced 5.6 nmol NO per gram of bacteria,
indicating that aerobic denitrification could be considered
an important source of NO. Inoculation of tomato
(Solanum lycopersicum Mill.) seedlings with both wt and
Faj009 induced LR and AR development. In contrast,
Faj164 mutant was not able to promote LR or AR when
seedlings grew in nitrate. When NO was removed with the
NO scavenger 2-(4-carboxyphenyl)-4,4,5,5,-tetramethylimidazoline-
1-oxyl-3-oxide (cPTIO), both LR and AR formation
were inhibited, providing evidence that NO mediated
Faj009 induced LR and AR development. In contrast,
Faj164 mutant was not able to promote LR or AR when
seedlings grew in nitrate. When NO was removed with the
NO scavenger 2-(4-carboxyphenyl)-4,4,5,5,-tetramethylimidazoline-
1-oxyl-3-oxide (cPTIO), both LR and AR formation
were inhibited, providing evidence that NO mediated
wild type (wt) and its mutants Faj009 (IAA-attenuated)
and Faj164 (periplasmic nitrate reductase negative), and
its correlation with tomato root-growth-promoting effects.
The wt and Faj009 strains produced 120 nmol NO per
gram of bacteria in aerated nitrate-containing medium. In
contrast, Faj164 produced 5.6 nmol NO per gram of bacteria,
indicating that aerobic denitrification could be considered
an important source of NO. Inoculation of tomato
(Solanum lycopersicum Mill.) seedlings with both wt and
Faj009 induced LR and AR development. In contrast,
Faj164 mutant was not able to promote LR or AR when
seedlings grew in nitrate. When NO was removed with the
NO scavenger 2-(4-carboxyphenyl)-4,4,5,5,-tetramethylimidazoline-
1-oxyl-3-oxide (cPTIO), both LR and AR formation
were inhibited, providing evidence that NO mediated
Faj009 induced LR and AR development. In contrast,
Faj164 mutant was not able to promote LR or AR when
seedlings grew in nitrate. When NO was removed with the
NO scavenger 2-(4-carboxyphenyl)-4,4,5,5,-tetramethylimidazoline-
1-oxyl-3-oxide (cPTIO), both LR and AR formation
were inhibited, providing evidence that NO mediated
architecture. In plants, nitric oxide (NO) mediates indole-
3-acetic acid (IAA)-signaling pathways leading to both lateral
(LR) and adventitious (AR) root formation. Here, we
analyzed aerobic NO production by A. brasilense Sp245
wild type (wt) and its mutants Faj009 (IAA-attenuated)
and Faj164 (periplasmic nitrate reductase negative), and
its correlation with tomato root-growth-promoting effects.
The wt and Faj009 strains produced 120 nmol NO per
gram of bacteria in aerated nitrate-containing medium. In
contrast, Faj164 produced 5.6 nmol NO per gram of bacteria,
indicating that aerobic denitrification could be considered
an important source of NO. Inoculation of tomato
(Solanum lycopersicum Mill.) seedlings with both wt and
Faj009 induced LR and AR development. In contrast,
Faj164 mutant was not able to promote LR or AR when
seedlings grew in nitrate. When NO was removed with the
NO scavenger 2-(4-carboxyphenyl)-4,4,5,5,-tetramethylimidazoline-
1-oxyl-3-oxide (cPTIO), both LR and AR formation
were inhibited, providing evidence that NO mediated
Faj009 induced LR and AR development. In contrast,
Faj164 mutant was not able to promote LR or AR when
seedlings grew in nitrate. When NO was removed with the
NO scavenger 2-(4-carboxyphenyl)-4,4,5,5,-tetramethylimidazoline-
1-oxyl-3-oxide (cPTIO), both LR and AR formation
were inhibited, providing evidence that NO mediated
wild type (wt) and its mutants Faj009 (IAA-attenuated)
and Faj164 (periplasmic nitrate reductase negative), and
its correlation with tomato root-growth-promoting effects.
The wt and Faj009 strains produced 120 nmol NO per
gram of bacteria in aerated nitrate-containing medium. In
contrast, Faj164 produced 5.6 nmol NO per gram of bacteria,
indicating that aerobic denitrification could be considered
an important source of NO. Inoculation of tomato
(Solanum lycopersicum Mill.) seedlings with both wt and
Faj009 induced LR and AR development. In contrast,
Faj164 mutant was not able to promote LR or AR when
seedlings grew in nitrate. When NO was removed with the
NO scavenger 2-(4-carboxyphenyl)-4,4,5,5,-tetramethylimidazoline-
1-oxyl-3-oxide (cPTIO), both LR and AR formation
were inhibited, providing evidence that NO mediated
Faj009 induced LR and AR development. In contrast,
Faj164 mutant was not able to promote LR or AR when
seedlings grew in nitrate. When NO was removed with the
NO scavenger 2-(4-carboxyphenyl)-4,4,5,5,-tetramethylimidazoline-
1-oxyl-3-oxide (cPTIO), both LR and AR formation
were inhibited, providing evidence that NO mediated
is its ability to modify plant root
architecture. In plants, nitric oxide (NO) mediates indole-
3-acetic acid (IAA)-signaling pathways leading to both lateral
(LR) and adventitious (AR) root formation. Here, we
analyzed aerobic NO production by A. brasilense Sp245
wild type (wt) and its mutants Faj009 (IAA-attenuated)
and Faj164 (periplasmic nitrate reductase negative), and
its correlation with tomato root-growth-promoting effects.
The wt and Faj009 strains produced 120 nmol NO per
gram of bacteria in aerated nitrate-containing medium. In
contrast, Faj164 produced 5.6 nmol NO per gram of bacteria,
indicating that aerobic denitrification could be considered
an important source of NO. Inoculation of tomato
(Solanum lycopersicum Mill.) seedlings with both wt and
Faj009 induced LR and AR development. In contrast,
Faj164 mutant was not able to promote LR or AR when
seedlings grew in nitrate. When NO was removed with the
NO scavenger 2-(4-carboxyphenyl)-4,4,5,5,-tetramethylimidazoline-
1-oxyl-3-oxide (cPTIO), both LR and AR formation
were inhibited, providing evidence that NO mediated
Faj009 induced LR and AR development. In contrast,
Faj164 mutant was not able to promote LR or AR when
seedlings grew in nitrate. When NO was removed with the
NO scavenger 2-(4-carboxyphenyl)-4,4,5,5,-tetramethylimidazoline-
1-oxyl-3-oxide (cPTIO), both LR and AR formation
were inhibited, providing evidence that NO mediated
wild type (wt) and its mutants Faj009 (IAA-attenuated)
and Faj164 (periplasmic nitrate reductase negative), and
its correlation with tomato root-growth-promoting effects.
The wt and Faj009 strains produced 120 nmol NO per
gram of bacteria in aerated nitrate-containing medium. In
contrast, Faj164 produced 5.6 nmol NO per gram of bacteria,
indicating that aerobic denitrification could be considered
an important source of NO. Inoculation of tomato
(Solanum lycopersicum Mill.) seedlings with both wt and
Faj009 induced LR and AR development. In contrast,
Faj164 mutant was not able to promote LR or AR when
seedlings grew in nitrate. When NO was removed with the
NO scavenger 2-(4-carboxyphenyl)-4,4,5,5,-tetramethylimidazoline-
1-oxyl-3-oxide (cPTIO), both LR and AR formation
were inhibited, providing evidence that NO mediated
Faj009 induced LR and AR development. In contrast,
Faj164 mutant was not able to promote LR or AR when
seedlings grew in nitrate. When NO was removed with the
NO scavenger 2-(4-carboxyphenyl)-4,4,5,5,-tetramethylimidazoline-
1-oxyl-3-oxide (cPTIO), both LR and AR formation
were inhibited, providing evidence that NO mediated
A. brasilense Sp245
wild type (wt) and its mutants Faj009 (IAA-attenuated)
and Faj164 (periplasmic nitrate reductase negative), and
its correlation with tomato root-growth-promoting effects.
The wt and Faj009 strains produced 120 nmol NO per
gram of bacteria in aerated nitrate-containing medium. In
contrast, Faj164 produced 5.6 nmol NO per gram of bacteria,
indicating that aerobic denitrification could be considered
an important source of NO. Inoculation of tomato
(Solanum lycopersicum Mill.) seedlings with both wt and
Faj009 induced LR and AR development. In contrast,
Faj164 mutant was not able to promote LR or AR when
seedlings grew in nitrate. When NO was removed with the
NO scavenger 2-(4-carboxyphenyl)-4,4,5,5,-tetramethylimidazoline-
1-oxyl-3-oxide (cPTIO), both LR and AR formation
were inhibited, providing evidence that NO mediated
Faj009 induced LR and AR development. In contrast,
Faj164 mutant was not able to promote LR or AR when
seedlings grew in nitrate. When NO was removed with the
NO scavenger 2-(4-carboxyphenyl)-4,4,5,5,-tetramethylimidazoline-
1-oxyl-3-oxide (cPTIO), both LR and AR formation
were inhibited, providing evidence that NO mediated
Solanum lycopersicum Mill.) seedlings with both wt and
Faj009 induced LR and AR development. In contrast,
Faj164 mutant was not able to promote LR or AR when
seedlings grew in nitrate. When NO was removed with the
NO scavenger 2-(4-carboxyphenyl)-4,4,5,5,-tetramethylimidazoline-
1-oxyl-3-oxide (cPTIO), both LR and AR formation
were inhibited, providing evidence that NO mediated
Azospirillum-induced root branching. These results show
that aerobic NO synthesis in A. brasilense could be
achieved by different pathways and give evidence for an
NO-dependent promoting activity on tomato root branching
achieved by different pathways and give evidence for an
NO-dependent promoting activity on tomato root branching
that aerobic NO synthesis in A. brasilense could be
achieved by different pathways and give evidence for an
NO-dependent promoting activity on tomato root branching
achieved by different pathways and give evidence for an
NO-dependent promoting activity on tomato root branching
-induced root branching. These results show
that aerobic NO synthesis in A. brasilense could be
achieved by different pathways and give evidence for an
NO-dependent promoting activity on tomato root branching
achieved by different pathways and give evidence for an
NO-dependent promoting activity on tomato root branching
A. brasilense could be
achieved by different pathways and give evidence for an
NO-dependent promoting activity on tomato root branching