IIB   20738
INSTITUTO DE INVESTIGACIONES BIOLOGICAS
Unidad Ejecutora - UE
artículos
Título:
Liver damageandcaspase-dependentapoptosisisrelatedtoprotein malnutrition inmice:Effectofmethionine
Autor/es:
CABALLERO, V.J.; MENDIETA, J.R.; LOMBARDO, D.; SACEDA, M.; FERRAGUT, J.A.; CONDE, R.D.; GIUDICI, A.M.
Revista:
ACTA HISTOCHEMICA
Editorial:
ELSEVIER GMBH, URBAN & FISCHER VERLAG
Referencias:
Lugar: ALEMANIA; Año: 2014
ISSN:
0065-1281
Resumen:
This studyaimedtodeterminewhethertheeffectsonthemouselivercausedbythreeperiodsoffeeding a protein-freedietfor5daysfollowedbyanormalcompletedietfor5days(3PFD-CD)arepreventedby a constantmethioninesupply(3PFD+Met-CD).TheexpressionsofcarbonicanhydraseIII(CAIII),fatty acid synthase(FAS),glyceraldehyde3-phosphatedehydrogenase(GAPDH)andglutathioneS-transferase P1 (GSTP1)wereassessedbyproteomicsandreversetranscriptase-polymerasechainreactions.Theliver redox statuswasexaminedbymeasuringtheactivitiesofsuperoxidedismutase(SOD)andcatalase(CAT), as wellasproteincarbonylation.Becauseoxidativestresscanresultinapoptosis,theactivityandcon- tent ofcaspase-3,aswellasthex-linkedinhibitoroftheapoptosisprotein(XIAP)andmitochondrial caspase-independent apoptosisinducingfactor(AIF)contentswereassessed.Inaddition,theliverhis- tomorphology wasexamined.Comparedtothecontrolsfedanormalcompletedietthroughout,feeding with 3PFD-CDincreasedtheFAScontent,decreasedtheCAIIIcontent,decreasedboththeSODandCAT activities, andincreasedproteincarbonylation.Italsoactivatedcaspase-3,decreasedtheXIAPcontent, decreased theAIFcontent,increasedthenumberofGSTP1-positivefociandcaspase-3-positivecells,and caused fattylivers.Conversely,thechangeswerelessenedtovaryingdegreesinmicefed3PFD+Met-CD. The presentresultsindicatethataregularMetsupplylessensthebiochemicalchanges,damage,and caspase-dependent apoptosisprovokedbyrecurrentdietaryaminoaciddeprivationinthemouseliver