IIB   20738
INSTITUTO DE INVESTIGACIONES BIOLOGICAS
Unidad Ejecutora - UE
artículos
Título:
Extracellular Phosphatidylinositol 4-phosphate is Associated to Lipoproteic Particles Present in Tomato Cell Suspensions and is Detected in Apoplastic Fluids of Tomato Plants.
Autor/es:
GONORAZKY, A. G.; LAXALT, A.M.; DEKKER, H.; REPM.; MUNNIK, T.; TESTERINK, C.; DE LA CANAL, L
Revista:
PLANT BIOLOGY
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Año: 2012 vol. 14 p. 41 - 49
ISSN:
1435-8603
Resumen:
proteins present in these pelleted 10 fractions. Most of these proteins have been previously characterized as having a role in defence responses. Next, we evaluated whether PI4P could also be detected in an entire plant system. For this, apoplastic fluids of tomato plants grown under basal conditions were analyzed by a lipid overlay assay. Interestingly, PI4P could be detected in intercellular fluids obtained from tomato leaflets and xylem sap of tomato plants. By 15 employing electrospray ionization tandem mass spectrometry (ESI-MS/MS) other phospholipids were found as well in intercellular fluids of tomato plants. These composed a markedly different profile from the phospholipid pattern identified in entire leaflets. Based on these results, the potential role of extracellular phospholipids in plant intercellular communication is discussed.