Identification and Expression of Stress-responsive

D IPPOLITO, S.; TERRILE, M. C.; GODOY, A.V.; CLAUDIA CASALONGUE; FIOL, D. F.

Foods V

Global Science

Año: 2011 vol. 5 p. 23 - 26

1749-7140

In order to identify genes involved in the potato (Solanum tuberosum L.) tuber response to the pathogenic fungus Fusarium solani f. sp. eumartii (F. eumartii) a differential screening of infected-potato cDNA library was carried out. In this work, we identified five potato cDNA clones coding for a heat shock protein, StHSP90 and four ribosomal proteins corresponding to small and large subunits, StS9, StS19 and StL3 StL36 respectively. Total RNA was extracted from non-wounding, wounding and wounding plus F. eumartii-infected tubers. The pattern of RNA accumulation for each gene was investigated by northern blot assays. Compared with control tubers, StHSP90 and StL36 genes were up-regulated by wounding and F. eumartii attack. StS9, StS19 and StL3 transcript levels showed a 2-fold increase upon F. eumartii infection. However, their expression profiles did not change by wounding. In addition StS9 transcript level was accumulated in salicylic acid- and jasmonic acid-treated leaves. In conclusion, our findings bring evidence on new different kinds of stress-responsive genes that may counteract F. eumartii attack in potato tubers. Putative functions for HSP90 and ribosomal proteins are discussed