INVESTIGADORES
MORCELLE DEL VALLE Susana Raquel
congresos y reuniones científicas
Título:
HEMOLYSIS AND ANTIHEMOLYSIS INDUCED BY ARGININE-BASED SURFACTANTS
Autor/es:
HERMET, M.; FAIT, M.E.; COMELLES, F.; CLAPÉS, P.; ALVAREZ, A.; PRIETO, E.; MATE, S.; VÁZQUEZ, R.F.; HERLAX, V.; MORCELLE, S.R.; BAKÁS, L.
Lugar:
Buenos Aires
Reunión:
Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017
Resumen:
Two novel arginine-based surfactants, Bz-Arg-NHC10 and Bz-Arg-NHC12, were characterized in terms of surface properties and interactionwith human red blood cells (HRBC) membranes. CMC values,Γmax (maximum surfactant adsorption at the air/liquid interface)and Amin (area per molecule) revealed better surface properties forBz-Arg-NHC12. The observation of cylindrical worm-like aggregatesof Bz-Arg-NHCn through atomic force microscopy (AFM) supportedthe predictions based on the surfactant packing parameter value(SPP). Erythrocyte membrane solubilization was induced by surfactantaggregates, since cell lysis was only evidenced at surfactantconcentrations above CMC. Changes in HRBC shape observed atdifferent surfactant concentrations allowed to conclude that a slowmechanism based on the insertion of surfactant monomers intothe HRBC membrane, followed by shedding of microvesicles isresponsible for the hemolysis produced by both surfactants at thelower concentrations tested. On the other hand, the extraction ofmembrane lipids upon collisions between HRBC and surfactant aggregatescompetes and prevents the microvesicles release for thehigher concentrations assayed.Moreover, we study the interaction of Bz-Arg-NHC12 with sheepred blood cells (SRBC) and HRBC due to their different membraneprotein/lipid composition. SRBC is a little more resistant than HRBCto the hemolytic effect of surfactant, but in both cases, the micellarform of the surfactant is the entity responsible of the hemolytic effect.As HC50, cAHmax value was higher than the CMC value. Thus, abiphasic behavior was observed for the surfactant studied, showinga wide range of protective concentrations when HRBC were tested,while for SRBC, the degree of protection of Bz-Arg-NHC12 wasabout 50% lower than for HBRC. However, only for SRBC treatedwith Bz-Arg-NHC12 a remarkable volume expansion was evidence,although no correlation with the antihemolytic potency was found.