Evaluation of zearalenone in vitro detoxification by Saccharomyces cerevisiae strains isolated from bovine forage

KELLER LAM; OLIVEIRA S; ABRUNHOSA L; ROSA CAR; KELLER KM,; DALCERO A.M.; CAVAGLIERI L.R.,; VENÂNCIO A

Conferencia; Global mycotoxin reduction strategies.; 2013

ISM-MycoRed

Zearalenone (ZEN) is a mycotoxin that has relatively low acute toxicity. However, it is a potent oestrogen that interferes with the reproductive tract of animals. Among other effects, ZEN decreases animals fertility, induces fibrosis in the uterus, breast cancer and endometrial carcinoma. Anti-mycotoxin additives (AMA) are defined as a group of products that, when added to animal feed, are capable to adsorb, inactivate, or to neutralize mycotoxins in the gastrointestinal tract of animals. An example of these products is an adsorbent based on yeast cell walls, a safe and beneficial animal feed additive. Yeast based products also act as a probiotic factor contributing to improve the general animal health because it stimulates their immune system and promotes the integrity of intestinal mucosa. Strains of Saccharomyces cerevisiae isolated from silage were tested for their zearalenone detoxification capability. Strains were growth grown on YPD supplemented with 2 μg/mL of the mycotoxin and its elimination from media was quantified over time by HPLC-FL. All the tested strains were able to reduce more than 90% of the mycotoxin in two days. The time required by the most active strains to reduce 50% and 90% of ZEN was 9 and 18 h, respectively. The less active strain needed 21 and 62 h, respectively. Este ensayo fue realizado a los tiempos que puede permanecer el alimento con el aditivo en el tracto gastrointestinal??? Sería interesante saber si entre los 10min y los 30 min existió reducción de ZEA??The metabolisation of ZEN into the more oestrogenic α-zearalenol was investigated.