Zearalenone, α- and β- zearalenol levels during the ensiling process in a laboratory maize silage model. zearalenone-binding/biotransforming ability of Saccharomyces cerevisiae strains isolated from silage.

GONZÁLEZ PEREYRA M.L., ; SULYOK M.,; BARALLA V.,; CHULZE S., ; KRSKA R,; DALCERO, A.,; CAVAGLIERI L.R.

Martina Franca

Conferencia; Global mycotoxin reduction strategies.; 2013

ISM-MycoRed

Silage is a major feed source for cattle in Argentina. Fusarium mycotoxins such as zearalenone (ZEA) and its toxic derivatives, α- zearalenol (α- ZOL) and β- zearalenol (β-ZOL) can contaminate maize before ensiling causing reproductive problems in animals. The aim of the present study was to monitor ZEA, α- ZOL and β-ZOL in artificially OK contaminated maize during the different stages of the silage formation, in a laboratory model, to determine the effect of the process and the indigenous microbiota on mycotoxin levels. In addition, two S. cerevisiae strains isolated from maize silage were tested for their ability to bind/biotransform ZEA in vitro. Twelve polystyrene bags were filled with previously contaminated (autoclaved 14-day Gibberella zeae Z3639 culture in rice) chopped whole-plant maize (2 kg each). Air was extracted with a vacuum cleaner and bags were sealed and stored in a dry and cool room and sampled at days 7, 45, 90, 120 and 127. Dry matter (DM%), moisture content (M%), water activity (aW), pH of samples were evaluated. Zearalenone, α- ZOL and β-ZOL levels were quantified by LC-MS/MS. Saccharomyces cerevisiae RC008 and RC009 (isolated from maize) were incubated shaking in PBS solution additioned with 299; 475.6 or 1,251 ng/ml ZEA for 1h at 25°C. Suspensions were centrifuged and unbound ZEA was quantified by LC-MS/MS. Chemical and physical analysis indicated silage maintained good quality and parameters did not vary (P