Optimization and production of probiotic antimycotoxin yeast biomass using bioetanol industry waste via Respose Surface Methodolgy

FOCHESATO AS, ; GALVAGNO MA, ; DOGI CA, ; CERRUTTI P, ; GONZÁLEZ PEREYRA ML, ; FLORES M, ; CAVAGLIERI L.

Advances in Biotechnology and Microbiology

Juniper Press

Año: 2018

2474-7637

Response surface methodology (RSM) was applied to optimize culture conditions for the production of probiotic and mycotoxin-binding Saccharomyces cerevisiae RC016 biomass for using alternative and low-cost substrates derived from the food industry such as dried distillers? grains and solubles (DDGS) and fermentable sugars sugarcane molasses (CMz). A significant influence of carbon source, nitrogen source, and vitamin source concentrations was evaluated by Plackett?Burman design (PBD). These factors were further optimized using the Box-Behnken design (BBD) of Response surface methodology. The highest growth rate (0.48h-1) was observed in the CMz medium, showing a 1.44 h duplication time. However, the higher productivity (0.57 g l-1 h-1) was observed in the DDGSE medium. CMz and DDGSE were low-cost substrates that performed significant kinetic and productive parameters for producing yeast biomass. The production of bulk amounts of RC016 S. cerevisiae strain biomass to be used in probiotic and mycotoxin-binding feed additives using low-cost industrial co-products as substrates was scaled-up and optimized. The selected culture conditions will improve RC016 S. cerevisiae production and application in feed additive formulations reducing production and commercialization costs of the final products in the market. The use of these agro-industrial wastes allow lower costs of large-scale production and contribute to sustainable development of the process.