Gliotoxin production by Aspergillus fumigatus strains from animal environment. Micro-analytical sample treatment combined with a LC-MS/MS method for gliotoxin determination.

PENA G.,; M.P. MONGE; GONZALEZ PEREYRA M.L.;; DALCERO A.M.,; ROSA C.A.; CHIACCHIERA S.M; CAVAGLIERI L.R.,

Mycotoxin research

Hans W. Schmidt

Lugar: Berlin; Año: 2015 vol. 31 p. 145 - 150

0178-7888

In this study, gliotoxin production by A. fumigatus strains from animal environment is studied. Moreover, a rapid, easy and environment friendly micro-analytical sample treatment procedure coupled with a LC-MS/MS method without a further clean-up for GLI determination is proposed. The gliotoxigenic ability of 143 strains was assayed in yeast extract sucrose medium and one millilitre of chloroform was used for toxin extraction without further clean-up. Mean recoveries at both spiking levels were 100.3 and 92.4%. Repeatability and within-laboratory reproducibility for different concentration levels ranged from 0.3 to 5.4 % and from 3.9 to 12.7, respectively. Most of the strains isolated from the different animal feeds produced gliotoxin at wide ranges. The micro-analytical sample treatment coupled with LC-MS/MS detection is a precise and useful methodology for determining GLI from fungal extracts of A. fumigatus and allows working both, fast and safely and also reducing the effect on the environment. This toxin plays a critical role in the pathobiology of A. fumigatus and its presence in animal environments could affect animal health and productivity; in addition, there are risks of contamination for rural workers during handling and storage of animal feedstuffs.