Effect of monogastric and ruminant gastrointestinal conditions on in vitro aflatoxin B1 adsorption ability by a montmorillonite.

MAGNOLI A.P., ; ALONSO V.A.,; CAVAGLIERI L.R.,; DALCERO A.M.,; CHIACCHIERA S.M.

FOOD ADDITIVES AND CONTAMINANTS

ELSEVIER

Año: 2013 vol. 30 p. 743 - 749

0265-203X

The main objective of this study was to evaluate the interference of the ions, ionic strength, ruminal fluid, and monensin in the adsorption capacity of aflatoxin B1 (AFB1) by a sodium bentonite (NaB) under simulated gastrointestinal conditions of monogastric and ruminant animals. The AFB1 adsorption isotherms showed a high affinity between the adsorbent and the toxin at each of assayed pHs The maximum affinities were observed at pH 4 and 6. On the other hand, the surface excesses at saturation were also affected by pH and presented the lower value at pH 4 and highest value at pH 2. A significant decrease in the surface excesses at saturation was observed in the presence of ionic strength and in the presence of MON. In the present study the presence of rumen fluid decreased only 25% the maximum adsorption capacity of the NaB. The assayed NaB showed a high affinity to adsorb AFB1 although different ions, ruminal fluid components and MON affected the adsorption capacity to bind AFB1. The environment present in the gastrointestinal tract, either monogastric or ruminant, could affect in vivo AFB1 adsorption by the bentonite.