The matrix influence on the adsorption levels of aflatoxin B1 and zearalenone by yeast cell wall.

PEREYRA C.M., ; WITTOUCK P., GENGHINI R., PEREYRA C.M., GONZÁLEZ-PEREYRA M.L., MAGNOLI C., CAVAGLIERI L.R., DALCERO A.M.; CHIACCHIERA S.M.,; ALONSO V.A.; MONGE M.P.; DALCERO A.M., CHIACCHIERA S.M.; CAVAGLIERI L.R.

JOURNAL OF APPLIED MICROBIOLOGY

WILEY-BLACKWELL PUBLISHING, INC

Año: 2013 vol. 114 p. 655 - 662

1364-5072

Aims: To evaluate the influence of the corn on the adsorption levels of aflatoxin B1 (AFB1) and zearalenone (ZEA) by yeast cell walls (YCW), in vitro. Methods and Results: Two commercial YCW were studied. The YCWs contains different percentages of polysaccharides. Yeast cell wall 1 and 2 contains 5.9% and 21% of mannans and 17.4% and 23% of β-glucans, respectively. Each YCW was resuspended at pH 2 and pH 6 solutions. Corn was used to study the matrix influence. An aliquot of 500 µL YCW concentration was added to each microtube containing 500 µL of 0.1, 0.25, 0.5, 1; 2.5 and 5 µg mL-1 AFB1 and 0.5, 5, 10, 20 and 50 µg mL-1 ZEA. Microtubes were introduced into a centrifuge with mechanical agitation at 37°C for 30 min and then centrifuged for 10 min at 14000 rpm; the supernatants were quantified by HPLC. The amount of bound toxin was plotted as a function of the amount of added toxin according to mathematical expressions proposed by three theoretical models. Both YCW were capable of adsorbing AFB1 and ZEA in amounts from 0.061 to 0.40 and from 0.10 and 0.26 g g-1, respectively. In the presence of the matrix, both adsorbents were not able of adsorbing AFB1. However, they could adsorb ZEA at levels from 0.03 to 0.23 g g-1. Conclusions: Both YCW adsorbed ZEA in the presence of corn and also under simulated gastrointestinal pH conditions. These results suggest that the studied YCW are potential candidates for ZEA adsorption. Significance and Impact of the Study: Several in vitro assays have informed the ability of different substrates including yeast walls to adsorb AFB1 and ZEA; none of them have evaluated their ability to adsorb AFB1 and ZEA in the presence of the corn. The matrix as corn can influence the adsorption phenomena of these mycotoxins.