In vitro study on the effect of Saccharomyces cerevisiae strains on growth and mycotoxin production by Aspergillus carbonarius and Fusarium graminearum.

ARMANDO M.R., ; DOGI C.C.,; POLONI V.,; ROSA C.A.R.,; DALCERO A.M.,; CAVAGLIERI L.R.

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2013 vol. 161 p. 182 - 188

0168-1605

The effect of Saccharomyces cerevisiae RC008 and RC016 strains, previously selected based on their aflatoxin B1 mycotoxin binding ability and beneficial properties, against Aspergillus carbonarius and Fusarium graminearum under different interacting environmental conditions was evaluated. In vitro studies on the lag phase, growth rate and ochratoxin A/zearalenone and DON production were carried out under different regimes of aw (0.95 and 0.99); pH (4 and 6); temperature (25 and 37°C) and oxygen availability (normal and reduced). Both yeast strains showed antagonistic activity and decreasing growth rate compared to the control. In general, the RC016 strain showed the greatest inhibitory activity. Except at the interacting condition 0.95aW, normal oxygen availability and 37°C, at both pH, Aspergillus carbonarius and Fusarium graminearum were able to produce large amounts of mycotoxins in vitro. In general, a significant decrease of mycotoxins levels in comparison with the control was observed. Saccharomyces cerevisiae RC008 and RC016 could be considered as effective agents to reduce growth and OTA, ZEA and DON production at different interacting environmental conditions, related to those found in stored feedstuff. The use of these strains with both beneficial and bio-control properties are important to be included in a novel product as additives of animal feed to control mycotoxigenic fungi in stored feedstuff.