Efficacy of corn silage inoculants on the fermentation quality under farm conditions and their influence on Aspergillus parasitucus, A. flavus and A. fumigatus determined by q-PCR.

DOGI CA, ; PELLEGRINO M,; POLONI V,; POLONI L,; PEREYRA CM,; SANABRIA A,; PIANZZOLA MJ,; DALCERO A,; ALONSO V.A.; MONGE M.P.; DALCERO A.M., CHIACCHIERA S.M.; CAVAGLIERI L.R.

FOOD ADDITIVES AND CONTAMINANTS

TAYLOR & FRANCIS LTD

Año: 2015 vol. 32 p. 229 - 235

0265-203X

Laboratory-scale silos were prepared to evaluate the efficacy of two different lactic acid bacteria (LAB) on the fermentation quality and mycobiota of corn silage moreover their influence on Aspergillus species variability by using the q-PCR technique was studied. Silage inoculated with Lactobacillus rhamnosus RC007 or L. plantarum RC009 were compared to uninoculated silage. Silos were opened after 1, 7, 45, 90 and 120 days after ensiling. At the end of the ensiling period, silos were left open for 7 days to evaluate aerobic stability. Rapid lactic acid production and decline in pH values were seen in the early stages of fermentation in silage inoculated with L. rhamnosus RC007. After aerobic exposure, a significant decline in lactic acid content was observed in untreated and L. plantarum RC009 inoculated silages. Counts for yeasted and toxigenic fungus remained lower, after aerobic exposure, in L. rhamnosus RC007-inoculated silage, in comparison with L. plantarum RC009 and uninoculated silages. Comparing the influence exerted by both BAL, it was observed that L. rhamnosus RC007 was more efficient inhibiting the three fungal species tested whose DNA concentrations, determinated by q-PCR, oscillated near the initial value (pre-ensiling maize). The ability of L. rhamnosus RC007 to a rapid lactic acid production and decline in pH values in the early stages of the fermentation along with the reduction of yeast and mycotoxicogenic fungus after aerobic exposure shows its potential as bio-control inoculant agent in animal feed.