INVESTIGADORES
CAVAGLIERI Lilia Renee
artículos
Título:
FUNGI AND AFLATOXIN B1 IN PRE AND POST-FERMENTED SORGHUM TRENCH TYPE SILOS DESTINED TO BOVINE INTENSIVE-REARING IN BRAZIL
Autor/es:
KELLER, LAM; PEREYRA, CM; CAVAGLIERI, LR; KELLER, KM; ALMEIDA, TX; DEVEZA, MV; ASSAD, RQ; ROSA, CAR
Revista:
Revista Bio ciencias
Editorial:
Revista Bio Ciencias Sistema Nacional de Investigadores (CONACyT-México)
Referencias:
Año: 2012 p. 81 - 91
ISSN:
2007-3380
Resumen:
The harmful effects of fungi in
sorghum silage are an important matter in
many countries with hot climates. This culture
is very susceptible to fungal contamination
and both the storage and processing of
this substrate are ideal for the development
of fungi there are able to produce mycotoxins.
The contamination with mycotoxins,
secondary products of the metabolism of
certain fungi, that promotes risks to both
animal and human health, causes numerous
losses for the country. The aim of this study
was to identify the toxicogenic fungi present
in sorghum silage for beef cattle consumption
and determine the occurrence of aflatoxin
B1 (AFB1) in the same substrate. A total
of 60 pre-fermented and 60 post-fermented
samples of sorghum silage were analyzed.
Total fungal counts and natural incidence
of toxigenic Aspergillus, Penicillium y Fusarium
species were performed on dichloran
rose bengal chloranphenicol agar, dichloran
glycerol agar 18 % and Nash-Snyder culture
media. Aflatoxin B1 contamination was
determined using high pressure liquid chromatography
(HPLC). About 30 % of samples
from pre-fermented sorghum and 55 %
of post-fermented samples were above the
recommended limits (1.0 x 104 CFU g-1). The
most frequent fungal species in both types
of sorghum samples were A. flavus, P. citrinum,
P. islandicum and F. verticillioides. An
average of 32 % samples, pre and post-fermented,
were positive for AFB1. The presence
of fungi and AFB1 in the feedstuffs indicates
contamination. This toxin could affect animal productivity and health. This fact requires periodic
monitoring to prevent the occurrence of
mycotoxicosis in animal production.
