INVESTIGADORES
CAVAGLIERI Lilia Renee
artículos
Título:
Fungal and mycotoxin contamination in mixed feeds: evaluating risk in cattle intensive rearing operations (feedlots)
Autor/es:
GONZÁLEZ PEREYRA M.L; CHIACCHIERA S.M; ROSA, C.A.R.; DALCERO, A.M.; CAVAGLIERI, L. R.
Revista:
Revista Bio ciencias
Editorial:
Revista Bio Ciencias, Universidad Autónoma de Nayarit
Referencias:
Año: 2012 p. 68 - 80
ISSN:
2007-3380
Resumen:
Argentina is the fourth global beef
producer. Exposure to mycotoxins through
contaminated feed is a major hazard for ruminants.
In the present study we assess
mycobiota, aflatoxin B1 (AFB1), fumonisin
B1 (FB1), deoxynivalenol (DON) and zearalenone
(ZEA) levels in total mixed rations
(TMRs) during two consecutive years. Total
fungal counts were evaluated and fungal
species were identified. Also, ability of A.
flavus isolates to produce AFB1 in vitro was
tested. Natural contamination with AFB1 and
FB1 was quantified by HPLC. Deoxynivalenol
and zearalenone were analysed by immunochromatography
and thin-layer chromatography
(TLC), respectively. Fungal counts varied
from not detectable (ND) to 2.10 x 108 CFU g-1.
The prevalent genera were Aspergillus spp
(60 %) and Fusarium spp (66.7 %), respectively.
The prevalent species was Aspergillus
fumigatus. 50 % of A. flavus strains produced
75 to 112.5 μg g-1 AFB1. 46 % of 2007 samples
were contaminated with 4 to 10 μg kg-1
AFB1. Deoxynivalenol was detected in 33.3 %
of the samples (≥ 1. 25 μg g-1). Fumonisin B1 and
ZEA were not detected. This study can be useful
to estimate the mycotoxicological risk of cattle
TMRs in this region and to compare results with
studies from other beef-producing countries.