Effect of gamma radiation on Aspergillus flavus and Aspergillus ochraceus ultrastructure and mycotoxin production.

J.M.M. RIBEIRO ; H. VITAL; C. MERKIS; A. CRISTOFOLINI ; M.B. MORLACO, L. SAGER, A. ASTORECA, M.L. GONZÁLEZ PEREYRA, L.R. CAVAGLIERI, A.M. DALCERO, C.A.R. ROSA.; C. MAGNOLI,; J. ORLANDO; M. CARÚ; A. DALCERO; L. CAVAGLIERI ; C.A.R. ROSA

RADIATION PHYSICS AND CHEMISTRY (OXFORD)

PERGAMON-ELSEVIER SCIENCE LTD

Año: 2011 vol. 80 p. 658 - 663

0969-806X

The aim of this work was to study the effect of gamma radiation (2 kGy) on Aspergillus flavus and Aspergillus ochraceus ultrastructure. Moreover, the influence on aflatoxin B1 and ochratoxin A production was also observed. Irradiated A. flavus strain showed a dull orangish colony while control strain showed the typical green colour. Minor differences were observed on stipes, metulae and conidia size between control and irradiated A. flavus and A. ochraceus strains. Irradiated fungi showed ultrastructural changes on cell wall, plasmalema and cytoplasm levels. The levels of mycotoxins produced by irradiated strains were two times greater than those produced by control strains. Successive transferences of irradiated strains on malt extract agar allowed the fungus to recuperate morphological characteristics. Although minor changes in the fungal morphology were observed, ultraestructural changes at cell wall level and the increase of mycotoxin production ability were observed. Inappropriate storage of irradiated food and feed would allow the development of potentially more toxicogenic fungal propagules.