Saccharomyces cerevisiae strains from animal environmental with aflatoxin B1 binding ability and anti-pathogenic bacteria influence in vitro

ARMANDO M.R.; DOGI C.A. ; PIZZOLITTO R.P.; ESCOBAR F. ; PEIRANO M.S. ; SALVANO M.A. ; SABINI L.I.; COMBINA M. ; DALCERO A.M. ; CAVAGLIERI L.R.

World Mycotoxin Journal

Wageningen

Año: 2011 vol. 1 p. 59 - 68

1875-0710

Mycotoxins are metabolites produced by fungi growing on foods or feeds and represent a serious hazard to humans and animals. Concerns related to the negative health impact of aflatoxins have lead to the investigation of strategies to prevent, eliminate or reduce the presence of these toxins in contaminated products. Saccharomyces cerevisiae strains are among promising candidates that can be applied in animal feed towards improving robustness of animals in the production environment. The aim of this work was to isolate and select S. cerevisiae strains from pig environment with aflatoxin B1 binding ability, able to tolerate gastrointestinal conditions and with some potential beneficial properties to the host. Saccharomyces cerevisiae strains were isolated from animal feed, faeces and gut and identified by morphological and molecular techniques. Aflatoxin B1 binding percentages varied among yeast strains according the AFB1 concentration used. The RC016 strain showed the highest adsorption percentage at the three AFB1 concentrations tested in this work (50; 100 and 500 ng/ml) followed by RC008 strain. All yeast strains were able to survive under gastrointestinal conditions and to strongly adhere to Vero cells. All S. cerevisiae strains showed co-aggregation with pathogenic bacteria (Escherichia coli, Enterobacter cloacae and Salmonella enterica sub sp. enterica). Only RC016 and RC008 strongly inhibited the three pathogens assayed. Saccharomyces cerevisiae strains RC016 and RC008 are promising microorganisms to be included in animal feed.