Aflatoxin B1 and fumonisin B1 in mixed cultures of Aspergillus flavus and Fusarium proliferatum on maize

MÓNICA PICCO, ANDREA NESCI, GERMÁN BARROS, LILIA CAVAGLIERI AND MIRIAM ETCHEVERRY

NATURAL TOXINS

WILEY-LISS

Lugar: Estados UNidos; Año: 2000 vol. 7 p. 331 - 336

1056-9014

Production of aflatoxin B1 and fumonisins B1 in pure and mixed cultures of Aspergillus flavus and Fusarium proliferatum were determined on irradiated maize seeds inoculated with different spore concentrations at 0.97 water activity (aW) and a temperature of 25°C. The highest levels of aflatoxin B1 were produced by A. flavus at the lowest levels of inoculum (103 spore ml-1). There was no spore concentration influence on fumonisins B1 production at 10, 20 and 35 days of incubation. When A. flavus was co-inoculated with F. proliferatum, aflatoxin B1 production was inhibited. The higher of the inocula levels of Fusarium produced, the higher the inhibition, and this inhibition increased during the incubation period. Total inhibition was reached at 35 days of incubation. There was no interaction influence on fumonisin B1 production at all inocula levels assayed. These results suggest that under optimal environmental conditions of substrate, water activity and temperature, the interaction between A. flavus and F. proliferatum could produce inhibition of aflatoxin B1 and inhibition of fumonisins B1.