Adsorption of ochratoxin A and zearalenone by potential probiotic Saccharomyces cerevisiae strains and its relation with cell wall thickness

ARMANDO MR; PIZZOLITTO RP; DOGI C.A.; A. CRISTOFOLINI; C. MERKIS; A. M. DALCERO; CAVAGLIERI LR

JOURNAL OF APPLIED MICROBIOLOGY

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2012 vol. 113 p. 256 - 264

1364-5072

Aims: to examine Saccharomyces cerevisae strains, with previously reported beneficial properties and aflatoxin B1 binding capacity, for their ability to reduce ochratoxin A and zearalenone and to study the relation between cell wall thickness and detoxificant ability of yeast strains. Methods and Results: mycotoxin binding assay at different concentrations and the effect of gastrointestinal conditions on mycotoxin binding were evaluated. Ultrastructural studies of yeast cells were performed by transmission electronic microscopy. All tested strains were capable to reduce ochratoxin A and zearalenone. Saccharomyces cerevisiae RC012 and RC016 showed the highest ochratoxin A reduction percentage, whereas RC009 and RC012 strains showed the highest zearalenone reduction percentages. Cell diameter/cell wall thickness relation showed a positive correlation between cell wall thickness and mycotoxin reduction ability. After exposure to gastrointestinal conditions, a significant increase in the mycotoxin binding was observed. Conclusions: all tested Saccharomyces cerevisiae were able to reduce ochratoxin A and zearalenone and a physical adsorption could be the main mechanism involved in mycotoxin reduction. Gastrointestinal conditions would enhance adsorption and not decrease the mycotoxin-adsorbent interactions. Significance and Impact of Study: live strains with mycotoxin binding ability and beneficial properties are potentially probiotics to be included in animal feed. Previous and the present results suggest that RC008 and RC016 strains are the most promising candidates for functional feed product development.