INVESTIGADORES
PASSEGGI Mario Cesar Guillermo
artículos
Título:
Effect of microplastics on activity of carboxylesterases and phosphatase digestive enzymes in scinax squalirostris tadpoles
Autor/es:
ATTADEMO, A.M.; CUZZIOL-BOCCIANI, A.P.; PELTZER, P.M.; FRANCO, V.G.; SIMONIELLO, M.F.; PASSEGGI (JR.), M.C.G.; LAJMANOVICH, R.C.
Revista:
ENVIRONMENTAL MONITORING AND ASSESSMENT
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2022 vol. 194 p. 71801 - 71810
ISSN:
0167-6369
Resumen:
Microplastics (MPs) are critical emerging pollutants around the world.There is a growing interest in the effects of MP ingestion, non-digestion and toxicity on aquatic organisms. Amphibian tadpoles are the vertebrate group that has received the least attention regarding this issue. The aim of the present study was to determine the ingestion of polyethylene MPs by Scinaxsqualirostris tadpoles by atomic force microscopy (AFM) and to evaluate the activities of carboxylesterase (CbE, using 4-naphthyl butyrate-NB-, and 1-naphthyl acetate-NA- as substrates) and alkaline phosphatase (ALP) under MP exposure. Enzyme activities were analyzed spectrophotometrically at 2 and10 days of exposure. Tadpoles were exposed to two different treatments during10 days: a negative control (CO, dechlorinated water) and MP (60 mg L-1). AFM images of the digestive contents of tadpoles revealed the presence of MPs.After 10 days of MP exposure, CbE (NB) activity was significantly higher and CbE (NA) activity was significantly lower in MP treatments than in controls. ALP activity decreased in MP treatments after 2 and 10 days of exposure. The detection of MP particles in the intestinal contents and the effects on metabolic enzymes in a common frog species evidence the potential health risk of MP to aquatic vertebrates. Thus, the differential response in enzymes and substrates demonstrate the need for considering the complex effects of contaminants and nutrients on ecosystems for ecotoxicological risk characterization.