CCT NOA SUR   20418
CENTRO CIENTIFICO TECNOLOGICO CONICET NOA SUR
Centro Científico Tecnológico - CCT
artículos
Título:
Potential applicability of chymotrypsin-susceptible microcin J25 derivatives to food preservation
Autor/es:
POMARES MF, SALOMÓN RA, PAVLOVA O, SEVERINOV K, FARÍAS RN AND VINCENT PA
Revista:
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Editorial:
American Society for Microbiology
Referencias:
Año: 2009
ISSN:
0099-2240
Resumen:
Microcin J25 (MccJ25) is a 21-residue ribosomally synthesized lariat peptide antibiotic. MccJ25 is active
against such food-borne disease-causing pathogens as Salmonella spp., Shigella spp., and Escherichia coli,
including E. coli O157:H7 and non-O157 strains. MccJ25 is highly resistant to digestion by proteolytic enzymes
present in the stomach and intestinal contents. MccJ25 would therefore remain active in the gastrointestinal
tract, affecting normal intestinal microbiota, and this limits the potential use of MccJ25 as a food preservative.
In the present paper, we describe a chymotrypsin-susceptible MccJ25 derivative with a mutation of Gly12 to Tyr
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
present in the stomach and intestinal contents. MccJ25 would therefore remain active in the gastrointestinal
tract, affecting normal intestinal microbiota, and this limits the potential use of MccJ25 as a food preservative.
In the present paper, we describe a chymotrypsin-susceptible MccJ25 derivative with a mutation of Gly12 to Tyr
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
present in the stomach and intestinal contents. MccJ25 would therefore remain active in the gastrointestinal
tract, affecting normal intestinal microbiota, and this limits the potential use of MccJ25 as a food preservative.
In the present paper, we describe a chymotrypsin-susceptible MccJ25 derivative with a mutation of Gly12 to Tyr
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
present in the stomach and intestinal contents. MccJ25 would therefore remain active in the gastrointestinal
tract, affecting normal intestinal microbiota, and this limits the potential use of MccJ25 as a food preservative.
In the present paper, we describe a chymotrypsin-susceptible MccJ25 derivative with a mutation of Gly12 to Tyr
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
including E. coli O157:H7 and non-O157 strains. MccJ25 is highly resistant to digestion by proteolytic enzymes
present in the stomach and intestinal contents. MccJ25 would therefore remain active in the gastrointestinal
tract, affecting normal intestinal microbiota, and this limits the potential use of MccJ25 as a food preservative.
In the present paper, we describe a chymotrypsin-susceptible MccJ25 derivative with a mutation of Gly12 to Tyr
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
present in the stomach and intestinal contents. MccJ25 would therefore remain active in the gastrointestinal
tract, affecting normal intestinal microbiota, and this limits the potential use of MccJ25 as a food preservative.
In the present paper, we describe a chymotrypsin-susceptible MccJ25 derivative with a mutation of Gly12 to Tyr
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
present in the stomach and intestinal contents. MccJ25 would therefore remain active in the gastrointestinal
tract, affecting normal intestinal microbiota, and this limits the potential use of MccJ25 as a food preservative.
In the present paper, we describe a chymotrypsin-susceptible MccJ25 derivative with a mutation of Gly12 to Tyr
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
present in the stomach and intestinal contents. MccJ25 would therefore remain active in the gastrointestinal
tract, affecting normal intestinal microbiota, and this limits the potential use of MccJ25 as a food preservative.
In the present paper, we describe a chymotrypsin-susceptible MccJ25 derivative with a mutation of Gly12 to Tyr
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
including E. coli O157:H7 and non-O157 strains. MccJ25 is highly resistant to digestion by proteolytic enzymes
present in the stomach and intestinal contents. MccJ25 would therefore remain active in the gastrointestinal
tract, affecting normal intestinal microbiota, and this limits the potential use of MccJ25 as a food preservative.
In the present paper, we describe a chymotrypsin-susceptible MccJ25 derivative with a mutation of Gly12 to Tyr
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
present in the stomach and intestinal contents. MccJ25 would therefore remain active in the gastrointestinal
tract, affecting normal intestinal microbiota, and this limits the potential use of MccJ25 as a food preservative.
In the present paper, we describe a chymotrypsin-susceptible MccJ25 derivative with a mutation of Gly12 to Tyr
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
present in the stomach and intestinal contents. MccJ25 would therefore remain active in the gastrointestinal
tract, affecting normal intestinal microbiota, and this limits the potential use of MccJ25 as a food preservative.
In the present paper, we describe a chymotrypsin-susceptible MccJ25 derivative with a mutation of Gly12 to Tyr
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
present in the stomach and intestinal contents. MccJ25 would therefore remain active in the gastrointestinal
tract, affecting normal intestinal microbiota, and this limits the potential use of MccJ25 as a food preservative.
In the present paper, we describe a chymotrypsin-susceptible MccJ25 derivative with a mutation of Gly12 to Tyr
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
including E. coli O157:H7 and non-O157 strains. MccJ25 is highly resistant to digestion by proteolytic enzymes
present in the stomach and intestinal contents. MccJ25 would therefore remain active in the gastrointestinal
tract, affecting normal intestinal microbiota, and this limits the potential use of MccJ25 as a food preservative.
In the present paper, we describe a chymotrypsin-susceptible MccJ25 derivative with a mutation of Gly12 to Tyr
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
present in the stomach and intestinal contents. MccJ25 would therefore remain active in the gastrointestinal
tract, affecting normal intestinal microbiota, and this limits the potential use of MccJ25 as a food preservative.
In the present paper, we describe a chymotrypsin-susceptible MccJ25 derivative with a mutation of Gly12 to Tyr
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
present in the stomach and intestinal contents. MccJ25 would therefore remain active in the gastrointestinal
tract, affecting normal intestinal microbiota, and this limits the potential use of MccJ25 as a food preservative.
In the present paper, we describe a chymotrypsin-susceptible MccJ25 derivative with a mutation of Gly12 to Tyr
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
present in the stomach and intestinal contents. MccJ25 would therefore remain active in the gastrointestinal
tract, affecting normal intestinal microbiota, and this limits the potential use of MccJ25 as a food preservative.
In the present paper, we describe a chymotrypsin-susceptible MccJ25 derivative with a mutation of Gly12 to Tyr
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
Salmonella spp., Shigella spp., and Escherichia coli,
including E. coli O157:H7 and non-O157 strains. MccJ25 is highly resistant to digestion by proteolytic enzymes
present in the stomach and intestinal contents. MccJ25 would therefore remain active in the gastrointestinal
tract, affecting normal intestinal microbiota, and this limits the potential use of MccJ25 as a food preservative.
In the present paper, we describe a chymotrypsin-susceptible MccJ25 derivative with a mutation of Gly12 to Tyr
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
present in the stomach and intestinal contents. MccJ25 would therefore remain active in the gastrointestinal
tract, affecting normal intestinal microbiota, and this limits the potential use of MccJ25 as a food preservative.
In the present paper, we describe a chymotrypsin-susceptible MccJ25 derivative with a mutation of Gly12 to Tyr
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
present in the stomach and intestinal contents. MccJ25 would therefore remain active in the gastrointestinal
tract, affecting normal intestinal microbiota, and this limits the potential use of MccJ25 as a food preservative.
In the present paper, we describe a chymotrypsin-susceptible MccJ25 derivative with a mutation of Gly12 to Tyr
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
present in the stomach and intestinal contents. MccJ25 would therefore remain active in the gastrointestinal
tract, affecting normal intestinal microbiota, and this limits the potential use of MccJ25 as a food preservative.
In the present paper, we describe a chymotrypsin-susceptible MccJ25 derivative with a mutation of Gly12 to Tyr
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
E. coli O157:H7 and non-O157 strains. MccJ25 is highly resistant to digestion by proteolytic enzymes
present in the stomach and intestinal contents. MccJ25 would therefore remain active in the gastrointestinal
tract, affecting normal intestinal microbiota, and this limits the potential use of MccJ25 as a food preservative.
In the present paper, we describe a chymotrypsin-susceptible MccJ25 derivative with a mutation of Gly12 to Tyr
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella enterica
12 to Tyr
that retained almost full antibiotic activity and efficiently inhibited the growth of pathogenic Salmonella entericaSalmonella enterica
serovar Newport and Escherichia coli O157:H7 in skim milk and egg yolk. However, unlike the wild-type
MccJ25, the MccJ25(G12Y) variant was inactivated by digestive enzymes both in vitro and in vivo. To our
knowledge, our results represent the first example of a rational modification of a microcin aimed at increasing
its potential use in food preservation.
MccJ25, the MccJ25(G12Y) variant was inactivated by digestive enzymes both in vitro and in vivo. To our
knowledge, our results represent the first example of a rational modification of a microcin aimed at increasing
its potential use in food preservation.
MccJ25, the MccJ25(G12Y) variant was inactivated by digestive enzymes both in vitro and in vivo. To our
knowledge, our results represent the first example of a rational modification of a microcin aimed at increasing
its potential use in food preservation.
MccJ25, the MccJ25(G12Y) variant was inactivated by digestive enzymes both in vitro and in vivo. To our
knowledge, our results represent the first example of a rational modification of a microcin aimed at increasing
its potential use in food preservation.
Escherichia coli O157:H7 in skim milk and egg yolk. However, unlike the wild-type
MccJ25, the MccJ25(G12Y) variant was inactivated by digestive enzymes both in vitro and in vivo. To our
knowledge, our results represent the first example of a rational modification of a microcin aimed at increasing
its potential use in food preservation.