PROBIEN   20416
INSTITUTO DE INVESTIGACION Y DESARROLLO EN INGENIERIA DE PROCESOS, BIOTECNOLOGIA Y ENERGIAS ALTERNATIVAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Synchrotron radiation XRF microprobe analysis applied to the study of As and Cu renal cortex accumulation after chronic arsenic intake
Autor/es:
PEREZ, CARLOS A; RUBATTO BIRRI, PAOLO N; PEREZ, ROBERTO D; CREMONEZZI, DAVID; RUBIO, MARCELO; BONGIOVANNI, GUILLERMINA A.
Lugar:
Karlsruhe, Germany
Reunión:
Congreso; 20th International Congress on X-ray Optics and Microanalysis (ICXOM20); 2009
Resumen:
The X-ray Fluorescence (XRF) analysis is a well-established method for quantitative multi-elemental
bulk analysis [1].When primary radiation is focused to micrometer dimensions by suitable optics, a nondestructive
and non-invasive method of analysis is obtained. The use of a synchrotron radiation source
allows constructing effective x-ray microprobes for study trace elements in small (nanogram) samples or
their distributions with high spatial resolution[2]. Since its operation, the XRF fluorescence beamline of
the LNLS has offered to the user community several hard x-ray microprobes configurations to develop
microscopic x-ray fluorescence analysis. Particularly interesting is the application of XRF microprobe
analysis to study biological systems [3]. It is known that arsenic (As) is one of the most abundant hazards
in the environment and it is a human carcinogen. Related you excretory function, the kidneys in humans,
animals models or naturally exposed fauna are target organs for As accumulation and deleterious effects.
Previous studies carried out by x-ray fluorescence analysis using synchrotron radiation (SR-ìXRF) showed
a high concentration of As in renal cortex from chronically exposed rats, suggesting that it is a suitable
model for studies on renal As accumulation [4]. This accumulation was accompanied by an important
increase in the copper (Cu) accumulation. The present study focused on the localization of these elements
along renal cortex and their correlation with physiological and histological As-related renal effects. This
study supports the hypothesis that tubular accumulation of As-Cu may have some bearing on the arsenicassociated
nephrotoxicological process.
a high concentration of As in renal cortex from chronically exposed rats, suggesting that it is a suitable
model for studies on renal As accumulation [4]. This accumulation was accompanied by an important
increase in the copper (Cu) accumulation. The present study focused on the localization of these elements
along renal cortex and their correlation with physiological and histological As-related renal effects. This
study supports the hypothesis that tubular accumulation of As-Cu may have some bearing on the arsenicassociated
nephrotoxicological process.
ìXRF) showed
a high concentration of As in renal cortex from chronically exposed rats, suggesting that it is a suitable
model for studies on renal As accumulation [4]. This accumulation was accompanied by an important
increase in the copper (Cu) accumulation. The present study focused on the localization of these elements
along renal cortex and their correlation with physiological and histological As-related renal effects. This
study supports the hypothesis that tubular accumulation of As-Cu may have some bearing on the arsenicassociated
nephrotoxicological process.