PROBIEN   20416
INSTITUTO DE INVESTIGACION Y DESARROLLO EN INGENIERIA DE PROCESOS, BIOTECNOLOGIA Y ENERGIAS ALTERNATIVAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Genomics and phylogeography of the Lager yeast parent: Saccharomyces eubayanus
Autor/es:
LIBKIND D.; EIZAGUIRRE J.; PERIS D.; BAKER E.C.; WANG B.; LOPES C.A.; RODRÍGUEZ M.E.; DE LOS RÍOS P.; HITTINGER C.
Reunión:
Jornada; V Jornadas Sudamericanas de Biología y Biotecnología de Levaduras; 2015
Resumen:
Saccharomyces eubayanus, the non-Saccharomyces cerevisiae parent of the lagerbrewingyeasts, known as Saccharomyces pastorianus syn. S. carlsbergensis wasoriginally isolated from Patagonian forests in Argentina. Later, genetically differentisolates were obtained from trees in USA and China. As a result several speculations onthe geographic origin of the Lager ancestor were generated and controversy existed onwhether a single or multiple hybridization events gave rise to the currently industriallyused Lager yeasts (Forhberg and Saaz groups). In this work, we summarize the currentknowledge on the worldwide distribution and tree association of S. eubayanus, withspecial emphasis on the patagonian area and Nothofagus forest. Multi-locus typing andgenomic data was used to determine the genetic structure of this species in Patagonia,compare with the sister and sympatric species Saccharomyces uvarum and relate witheco-geological factors. The different molecular markers used showed the existence inPatagonia of two major lineages that share habitat only above latitude 43° and possesssubstantial internal genetic divergence. Moreover, based on new high-quality genomedata for the type strain CRUB 1568T and through comparative analyses with the genomeof multiple Lager yeasts we provide insights on the evolutionary history of thesedomesticated allopolyploid hybrid that makes Lager beer possible.