PROBIEN   20416
INSTITUTO DE INVESTIGACION Y DESARROLLO EN INGENIERIA DE PROCESOS, BIOTECNOLOGIA Y ENERGIAS ALTERNATIVAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Chlorpyrifos produces dissimilar effects on proliferation in breast cancer cells
Autor/es:
VENTURA, C; NÚÑEZ, M; GAIDO, V; UBERTONE CORRALES, V; MARTINEL LAMAS, D; VENTURINO A; RANDI, A; RIVERA, E; COCCA, C
Lugar:
Puerto Madryn
Reunión:
Congreso; XLVI Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2010
Institución organizadora:
SAIB
Resumen:
A large body of literature suggests that environmental chemicals are involved in breast carcinogenesis. It has been postulated the use of the organophosphorus chlorpyrifos for replacing the current pesticide for fruit-bearing trees treatment in Alto Valle de Rio Negro and Neuquen. The aim of the present study was to evaluate the effect of chlorpyrifos on cell proliferation of human breast cancer cell line, MCF-7. Proliferation was evaluated by clonogenic assay and bromodeoxyuridine (BrdU) incorporation; cell viability by MTT assay. Reactive oxygen species (ROS) were determined using DCF fluorescent staining and flow citometry. Protein expression was assessed by Western blot. Our results indicate that high concentrations of chlorpyrifos (50 ìM) decreased 40% clonogenic proliferation and 18% cell viability. The inhibitory effect on proliferation was associated to an increase of 32% in the content of ROS and 48% p27 protein expression. On the other hand, exposure of cells to low concentrations of chlorpyrifos (50 nM) stimulated BrdU incorporation in 88%. This effect was related to an enhanced expression of proliferating cell nuclear antigen (PCNA) and cyclin E. We conclude that chlorpyrifos modulates proliferation in a dose dependent way, increasing proliferation at low doses while decreasing it at high concentrations.