PROBIEN   20416
INSTITUTO DE INVESTIGACION Y DESARROLLO EN INGENIERIA DE PROCESOS, BIOTECNOLOGIA Y ENERGIAS ALTERNATIVAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Chlorpyrifos produces dissimilar effects on proliferation in breast cancer cells
Autor/es:
VENTURA, C; NÚÑEZ, M; GAIDO, V; UBERTONE CORRALES, V; MARTINEL LAMAS, D; VENTURINO A; RANDI, A; RIVERA, E; COCCA, C
Lugar:
Puerto Madryn
Reunión:
Congreso; XLVI Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2010
Institución organizadora:
SAIB
Resumen:
A large body of literature suggests
that environmental chemicals are
involved in breast carcinogenesis.
It has been postulated the use of
the organophosphorus chlorpyrifos
for replacing the current
pesticide for fruit-bearing trees
treatment in Alto Valle de Rio Negro
and Neuquen. The aim of the present
study was to evaluate the effect
of chlorpyrifos on cell
proliferation of human breast cancer cell line,
MCF-7. Proliferation was evaluated
by clonogenic assay and
bromodeoxyuridine (BrdU)
incorporation; cell viability by MTT
assay. Reactive oxygen species (ROS)
were determined using DCF
fluorescent staining and flow
citometry. Protein expression was
assessed by Western blot. Our
results indicate that high
concentrations of chlorpyrifos (50
ìM) decreased 40% clonogenic
proliferation and 18% cell
viability. The inhibitory effect on
proliferation was associated to an
increase of 32% in the content of
ROS and 48% p27 protein expression.
On the other hand, exposure
of cells to low concentrations of
chlorpyrifos (50 nM) stimulated
BrdU incorporation in 88%. This
effect was related to an enhanced
expression of proliferating cell
nuclear antigen (PCNA) and cyclin
E. We conclude that chlorpyrifos
modulates proliferation in a dose
dependent way, increasing
proliferation at low doses while
decreasing it at high
concentrations.