Patagonian red wines: selection of Lactobacillus plantarum isolates as potential starter cultures for malolactic fermentation

BÁRBARA MERCEDES BRAVO FERRADA; AXEL HOLLMANN; LUCRECIA DELFEDERICO; DANAY VALDÉS LA HENS; ADRIANA CABALLERO ; LILIANA SEMORILE

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

SPRINGER

Lugar: Berlin; Año: 2013 p. 1 - 13

0959-3993

The aim of this study was to evaluate fifty three Lactobacillus plantarum isolates obtained from a Patagonian red wine, molecularly identified and typified using RAPD analysis, in order to select starter cultures for malolactic fermentation (MLF). The results obtained suggest a considerable genetic diversity, taking into account that all Lb. plantarum isolates were obtained from one cellar and one vintage. Based on the capacity to tolerate a concentration of 14% ethanol in MRS broth for two days, eight isolates were selected for the subsequent analysis. The incidence of various wine stress factors (ethanol, acid pH, lysozyme and sulfur dioxide) on isolates growth was studied. Besides, glucosidase and tannase activities were evaluated, and the presence of genes involved in the synthesis of biogenic amines was examined by PCR. A previously characterized indigenous Oenococcus oeni strain was included with comparative purposes. Differences in technologically relevant characteristics were observed among the eight Lb. plantarum selected 31 isolates, revealing an isolate-dependent behavior. Detectable glucosidase and tannase activities were found in all isolates. The presence of genes encoding histidine and tyrosine descarboxylases and putrescine