PROBIEN   20416
INSTITUTO DE INVESTIGACION Y DESARROLLO EN INGENIERIA DE PROCESOS, BIOTECNOLOGIA Y ENERGIAS ALTERNATIVAS
Unidad Ejecutora - UE
artículos
Título:
Chlorpyrifos modulates estrogen-dependent and -independent human breast cancer cell lines proliferation
Autor/es:
VENTURA, C; NÚÑEZ, M; MIRET, N.; MARTINEL LAMAS, D; RANDI, A; VENTURINO A; RIVERA, E; COCCA, C
Revista:
TOXICOLOGY LETTERS
Editorial:
ELSEVIER IRELAND LTD
Referencias:
Lugar: Amsterdam; Año: 2012 vol. 213 p. 184 - 193
ISSN:
0378-4274
Resumen:
It has reported that many environmental compounds may
display estrogenic actions and these findings led to researchers to associate
breast cancer risk with the use of some pesticides. The aim of this work was to
investigate the effect of chlorpyrifos (CPF) on cell proliferation and the ERa-dependence of this action employing
MCF-7 and MDA-MB-231 breast cancer cell lines. We have also analyzed CPF action
on the cell cycle distribution and the cyclins that are implicated in G1-S and
intra-S checkpoints. Finally, the action on cell death and ROS production were
studied. We demonstrated the ability of CPF 0.05 uM to induce
cell proliferation through ERa in
hormone-dependent breast cancer cells. In contrast, CPF 50 uM
induces intra-S arrest modifying checkpoints proteins, through a mechanism that
may involve changes in redox balance in MCF-7. In MDA-MB-231, we have found
that CPF 50 uM produces an arrest in G2/M phase which could be
related to the capacity of the pesticide for binding to tubulin sites altering
microtubules polymerization. Altogether, our results provide new evidences on
the action of the pesticide CPF as an environmental breast cancer risk factor
due to the effects that causes on the mechanisms that modulate breast cell
proliferation.