Oenococcus oeni from patagonian red wines: isolation, characterization and technological properties

BRAVO FERRADA B.,; DELFEDERICO L.; HOLLMANN A.; VALDÉS LA HENS D.; CURILÉN Y.; CABALLERO A.; SEMORILE L.

International Journal of Microbiology Research

Bioinfo Publications

Año: 2011 vol. 3 p. 48 - 55

0975-5276

Pinot noir vinifications carried out at industrial scale during 2008 vintage were monitored in Patagonian region, Argentina, and several lactic acid bacteria were obtained. By analysis of a fragment of rpoB gene, speciesspecific PCR of malolactic enzyme and sequencing of 16S rRNA gene, three isolates were identified as Oenococcus oeni. Polyphasic typing made by carbohydrate fermentation behaviour and RAPD-PCR grouped the isolates and O. oeni reference strain in three different clusters. Additionally, the effect of ethanol on bacterial growth and malolactic activity was evaluated. In spite of some inhibitory effects were found, the three isolates were able to growth in all ethanol concentration tested. A clear correlation was found between the different clusters obtained and their ethanol tolerance. All indigenous O. oeni showed a strong malolactic activity at all ethanol concentrations assayed, indicating that this alcohol just affect the growing parameters of bacteria, but not the malolactic activity. These promissory results suggest a potential enological application of the O. oeni indigenous isolates as malolactic fermentation starters in winemaking.