Mapping Acidithiobacillus populations with high resolution onto a natural environmental gradient.

OLAVE, I.; GIAVENO A,; DIAZ, M.; QUATRINI R.; ULLOA R.; CUEVAS M; TEMPORETTI, P.; ISSOTTA F; P CHIACHARINI,; PEDROZO FL; JHONSON D.

AMSTERDAM

Congreso; ISME 2019; 2019

In this study, the microdiversity of Acidithiobacillus populations inhabiting the Copahue-Caviahue-ChanchoCo geothermal system is analysed using culture-dependent and independent strategies. Genomic DNA extracted from representative isolates and environmental DNA obtained from diverse sampling points along the river, was used to PCR-amplify the 16S rRNA gene marker.After high throughput sequencing, phylogenetic and oligotyping analyses were used to infer the taxonomy of the representative isolates and sequence clones, and to resolve meaningful differences between closely related populations along the environmental gradient. The correlationbetween the emerging population structure and the physic-geochemical gradient parameters is analysed through multivariate statistical analysis in order to gain insights into the ecological and  evolutionary processes that structure and maintain microbial diversity in this ecosystem.