CONICET | Buscador de Institutos y Recursos Humanos

Although contamination and invasive species are two of the most relevant anthropogenic drivers affectingecosystems, their joint impact on the environment has been poorly investigated. Glyphosate, directly or indirectly,contaminates freshwater systems which in turn may be invaded by the golden mussel Limnopernafortunei. Under laboratory conditions, we studied the combined effect of technical-grade glyphosate, RoundupMax® and Glifosato Atanor®, in scenarios with and without L. fortunei, on phytoplankton from Salto GrandeReservoir (Uruguay River, Argentina). We expected that the effects of the interaction on phytoplankton andwater quality would vary with the form of herbicide applied. The assay was conducted for 14 days (Tf) using 3-Lbottles as experimental units. Eight treatments were performed in triplicate: C: Control; M: mussel; G: technicalgradeglyphosate acid; R: Roundup Max®; A: Glifosato Atanor®; MG: mussel +technical-grade glyphosate acid,MA: mussel +Glifosato Atanor® and MR: mussel +Roundup Max®. The active ingredient was applied at 6 ppm.The dissipation of glyphosate in water was 1.5?2.6 times higher in presence of mussels. Treatments G and Ashowed an increase in phytoplankton abundance, mainly the cyanobacteria Microcystis spp. wich rised to 289%and 639% at Tf, respectively, relative to their values at Ti. Roundup Max® limited the growth of Microcystis spp.,as its abundance decreased 59% relative to Ti. L. fortunei reduced phytoplankton abundances at Tf. Evennessincreased significantly in M, MG, MR and MA, while it decreased in G, R and A relative to C. The interaction offactors produced a significant synergistic increase in periphyton; periphytic chlorophyll a concentration was0.81 ± 0.02 μg cm−2 for MR; 0.09 ± 0.02 μg cm−2 for MA and 0.02 ± 0.01 μg cm−2 for MG. Limnopernafortunei appeared as the driving force in the interaction. The assay described here allows for the rapid assessmentof the impact of these types of agents on freshwater