INIBIOMA   20415
INSTITUTO DE INVESTIGACIONES EN BIODIVERSIDAD Y MEDIOAMBIENTE
Unidad Ejecutora - UE
artículos
Título:
Oldies (but goldies!): extracting DNA from cryopreserved allozyme homogenates
Autor/es:
ARBETMAN M & A.C. PREMOLI
Revista:
JOURNAL OF HEREDITY
Editorial:
OXFORD UNIV PRESS INC
Referencias:
Año: 2011 p. 764 - 769
ISSN:
0022-1503
Resumen:
In this work, we describe a method that allows the
extraction of plant genomic DNA from frozen enzyme
homogenates. To test if reliable DNA was recovered from
allozyme extracts, we compared these results with that
obtained from fresh frozen and dried material. Sequencing
and microsatellite fragments were analyzed for samples
from 5 plant families in order to check for chloroplast and
nuclear DNA quality. High-molecular weight DNA was
recovered from frozen homogenates, and it was found that
the quality was independent of storage time, which varied
from 1 to 9 years. High-quality PCR products along with
sequences and microsatellite fragments were obtained for
nearly all analyzed samples. Frozen protein homogenates
from previous population studies could thus be used for
further phylogeographical and phylogenetic analyses using
DNA molecular tools. This simple method provides an
opportunity to take advantage of stored cryopreserved
samples.