IBCN   20355
INSTITUTO DE BIOLOGIA CELULAR Y NEUROCIENCIA "PROFESOR EDUARDO DE ROBERTIS"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Bacterial Lipopolysaccharide-induced protection against light-induced retinal damage in rats.
Autor/es:
BORDONE M., LANZANI F., LÓPEZ JJ., ROSENSTEIN R.
Lugar:
Huerta Grande, Códoba
Reunión:
Congreso; II Reunión Conjunta de Neurociencias, XXV Reunión de la Sociedad Argentina de Investigación en Neurociencias (SAN) y XII Taller Argentino de Neurociencias (TAN); 2010
Institución organizadora:
Sociedad Argentina de Investigación en Neurociencias (SAN)
Resumen:
We
analyzed the effect of lipopolysaccharide (LPS) on Light-induced retinal
damage. Male Wistar rats were intravitreously injected with LPS in one eye and
vehicle in the fellow eye, one day befote an intense Light exposure for 24 h.
Electroretinograms (ERGs) were
registered at different time points alter Light exposure, and retinal histology
was examined 14 days days alter Light. Apoptosis was examined through the
assesment of mitochondrial bax/citosolic bax ratio, and lipid peroxidation was
evaluated through TBARS method. Animals were injected with an iNOS inhibitor
(aminoguanidine, AG) or a mitochondrial K+ ATP channel blocker
(5-hydroxidecanoic acid, 5HD), or a phosphatidylinositol 3-kinase (PI3K)
inhibitor (wortmannin, WT) and electroretinographic and histologic análisis
were performed 7 days alter Light exposure. Light significantly decreased the ERG a- and b- wave amplitude, and the
total retina, photoreceptor segments, outer nuclear layer thickness, and the
number of cells in ganglion cell layer, whereas LPS significantly prevented
these alterations. Light exposure increased the mitochondrial bax/ cytosolic
bax ratio and TBARS levels, whereas LPS prevented these changes. This injection
of WT (but not of AG or 5HD) completely blocked the functional and histological
protection.