Effects of intranasal delivery of human recombinant erytropoieti (rHuEpo) on spontaneous motor activity in hypoxic rats

MERELLI A, CALTANA L, RAMOS AJ, LAZAROWSKI A, BRUSCO A.

Santorini

Conferencia; 5th Santorini Conference Biologie Prospective; 2010

Stroke is a major human health problem inducing long-term dissability without efficient therapeutic options. Under hypoxia, hte hypoxia-inducible factor-1a (HIF1a) activates several genes as Erytropoietin receptor (Epo-R) related with O2 supply, and the multidrug-resistence gene (MDR-1) related with drug refractory phenotype. We have described that direct brain cortical injection of Cobalt Chloride (CoCl2) produces focal hypoxia-like lesion, wiwth neuronal and glial alterations. HIF-1a stabilization and MDR-1 overexpression., Intranasal (IN) drugs delivery can bypasses teh MDR-1 expressed blood-brain barrier. We evaluated the effects of IN-rHuEpo administration on spontaneous motor activity (SMA) and the brain pattern expression of HIF-1a, MDR-1, and Epo-R in our cobalt-induced brain hypoxia model.