IBCN   20355
INSTITUTO DE BIOLOGIA CELULAR Y NEUROCIENCIA "PROFESOR EDUARDO DE ROBERTIS"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Study of behavior and the BDNF signaling pathway in hyposerotonergic Pet1-/- mice.
Autor/es:
FOLTRAN, ROCÍO B.; MAROTEAUX, LUC; BÉCHADE, CATHERINE; SCOTTO-LOMASSESE, SOPHIE; DIAZ, SILVINA L.
Lugar:
Virtual
Reunión:
Congreso; XXXV Congreso Anual de la Sociedad Argentina de Investigación en Neurociencias (SAN); 2020
Institución organizadora:
Sociedad Argentina de Investigación en Neurociencias
Resumen:
Modulation of serotonergic neurotransmission has revealed as an exciting tool to study the process of neurogenesis in the adult hippocampus (HC). Different models of hyposerotonergic mice show enhanced survival of newborn neurons in the HC, with no change in proliferation. In Pet1-/- mice most of serotonergic neurons do not differentiate, leading to an 80 % depletion of serotonin. We wondered if the supernumerary neurons in these mice could modulate certain behaviors. Compared to their respective control group, young adult male Pet1-/- mice showed a tendency to an increased compulsive behaviour in the Nestlet® shredding test, but no effect was seen in the Marble Burying test. To study the role of the new neurons in the HC, we also conducted the Object Pattern Separation (OPS), a test that allows finding subtle differences compared to classical PS tests. Similar to what is reported for other models of enhanced neurogenesis, the hyposerotonergic mice showed a better discrimination index than control mice. As the brain derived neurotrophin factor (BDNF) signaling pathway is linked to neuron survival, BDNF isoforms and their receptors were analyzed in the HC by Western blot and RT-qPCR. Proteins and RNA were extracted from hippocampi and levels of BDNF, TrkB, p75, and proBDNF were quantified. We couldn?t observe any differences in protein levels, compared to their respective controls. In contrast, when analyzing the expression of the different transcripts of the BDNF gene, we observed a significant increase in the expression of the transcript VI. Our results show that this hyposerotonergic mice model is similar in their behavior, both in compulsivity tests and in their ability for pattern separation, to other serotonin-depleted animal models described in the bibliography. On the other hand, the BDNF pathway could be involved in the regulation of neurogenesis when serotonin is depleted.