Changes in alternative splicing of GluN1 NMDAR subunit in a GluN2A KD model

MARÍA FLORENCIA ACUTAIN; MARÍA LAURA GOBBINI; MARÍA VERÓNICA BAEZ; MARÍA PILAR CANAL

Congreso; XXXV Congreso Anual de la Sociedad Argentina de Investigación en Neurociencias; 2020

Sociedad Argentina de Investigación en Neurociencias

NMDAR receptor (NMDAR) is a heterotetramer composed of two obligatory subunits, GluN1; and two regulatory subunits, GluN2 (A to D) or GluN3 (A and B). Alternative splicing of GluN1 mRNA produces eight splice variants that alternate in, N1 cassette at the N terminal or, concerning the C-terminal, GluN1 variants are due to the presence or absence of C1, C2 and C2? cassettes.Previous works have described that knock-down (KD) of GluN2A regulatory subunit produces alterations in expression of GluN1. In this work, we analyse if changes in GluN1 expression in GluN2A KD are related to changes in NDMAR subcellular localization and also, if these changes are associated to modifications in NMDAR splicing variants. Preliminary results showed that N1 cassette inclusion does not change due to GluN2A KD, nonetheless, we found changes in the alternative splicing of C-terminus cassettes. Furthermore, we observed changes in GluN1 levels both at soma and dendrites. Although these are preliminary results, they suggest that changes in GluN2A modulates the NMDAR localization and the alternative splicing of GluN1.