IBCN   20355
INSTITUTO DE BIOLOGIA CELULAR Y NEUROCIENCIA "PROFESOR EDUARDO DE ROBERTIS"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
GluN2A-NMDAR hippocampal KD increases seizure susceptibility without changes in LTM
Autor/es:
MARIA FLORENCIA ACUTAIN; LUCAS DE OLIVEIRA ALVARES; DIANA A. JERUSALINSKY; JORDANA GRIEBLER LUFT; MARIA V. BAEZ
Reunión:
Congreso; Reunión Anual de la Sociedad Argentina de Investigación en Neurociencias (SAN) 2020; 2020
Institución organizadora:
Sociedad Argentina de Investigación en Neurociencias
Resumen:
Epilepsy represents one of the more complex and misunderstanding neurological disorders characterized by recurrent unprovoked seizures which lead to aberrant firing in brain circuits. Furthermore, mutations in NMDA receptor (NMDAR) subunits were related to Epilepsy. In cognitive related brain structures, like hippocampus, GluN2A and GluN2B subunits are the NMDAR most expressed regulatory subunits, and undergoes a tightly regulation. It was shown that the balance in these synaptic GluN2-type subunits is responsible for adequate glutamatergic neurotransmission, which is altered in several neurological disorders. In order to better understand the role of GluN2A in synaptic plasticity and behavior, we induce a knock-down (GluN2A-KD) in the CA1 region of young adult Wistar rats and perform several tasks. We evaluate locomotion, spatial exploration, associative (novel object recognition) and emotional memory (fear conditioning), as well as anxiety like parameters to characterize the KD model. Furthermore, we test seizure induced susceptibility in those rats. Our results showed that LTM was unaltered in GluN2A KD rats. Nevertheless, these animals were more susceptible to reach the stratus epilepticus than controls. These results suggest that GluN2A down regulation, and also, the imbalance in synaptic GluN2-type subunits, could facilitate seizure outcome that is a hallmark in several types of Epilepsy.