IBCN   20355
INSTITUTO DE BIOLOGIA CELULAR Y NEUROCIENCIA "PROFESOR EDUARDO DE ROBERTIS"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
SHIGA TOXIN 2 (STX2) AND LPS FROM ENTEROHEMORRHAGIC ESCHERICHIA COLI (EHEC) PRODUCE A TLR4-INDEPENDENT MICROGLIAL REACTIVITY AND PRO-INFLAMMATORY CYTOKINES
Autor/es:
VILLARREAL A; GEOGHEGAN PA; GOLDSTEIN RJ; ELIZAGARAY LF; PINTO A; CELI AB; CANGELOSI A; RAMOS AJ
Reunión:
Congreso; Reunión anual de sociedades de biociencias 2020. Sociedad Argentina de Investigaciones Clínicas; 2020
Institución organizadora:
Sociedad Argentina de Investigación Clínica (SAIC)
Resumen:
Stx2 from EHEC is the main cause of hemolytic uremic syndromethat is defined clinically by microangiopathic hemolytic anemia,thrombocytopenia, and acute renal failure. Stx2 can also lead toencephalopathy with motor, cognitive and emotional impairmentsin 30% of cases. We have studied that Stx2 can bind to neuronsthrough its receptor Gb3. Other authors have reported that Stx2 maybind to TLR4 in leukocytes, which leads to its activation and cytokinerelease. Given that microglial cells (MC) are CNS resident macrophages, we hypothesize that MC would respond to Stx2 through aTLR4 receptor. Therefore, our aim was to determine by in vivo andin vitro studies whether Stx2 produces MC activation through TLR4and cytokine release. Mice were subjected to the following sublethaltreatments: vehicle (control) or Stx2 (3ng)+LPS (800ng), to determine MC reactivity by immunofluorescence and cytokine releaseby flow cytometry. In vitro assays of MC were obtained from TLR4KO rat brains which were treated with either control, LPS (50ng/ml), Stx2 (50 or 200ng/ml) or Stx2+LPS. One way ANOVA analysis,and Bonferroni post-hoc analysis were performed for in vivo and invitro studies. After 24h of treatment, Stx2+LPS treated mice showedan increase in the expression of IBA1 as well as in the number ofMC (p