IBCN   20355
INSTITUTO DE BIOLOGIA CELULAR Y NEUROCIENCIA "PROFESOR EDUARDO DE ROBERTIS"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Beta1 integrin is necessary for retinal ganglion cell axon growth mediated by EphA3.
Autor/es:
RONDON, JONATHAN; FIORE, LUCIANO; MEDORI, MARA; ROBALDO, MANUEL; SPELZINI, GONZALO; SCICOLONE, GABRIEL
Reunión:
Congreso; SAN 2020. XXXV Virtual Meeting; 2020
Institución organizadora:
Sociedad Argentina de Investigacion en Neurociencias
Resumen:
Beta1 integrin is necessary for retinal ganglion cell axon growth mediated by EphA3.MARA MEDORI*1,2; JONATHAN RONDON1,2; GONZALO SPELZINI1,2; MANUEL ROBALDO1,2; LUCIANO FIORE1,2; GABRIEL SCICOLONE1,2.1. CONICET ? Universidad de Buenos Aires, Instituto de Biología Celular y Neurociencias ?Prof. E. De Robertis? (IBCN). Ciudad de Buenos Aires, Argentina.2, Universidad de Buenos Aires, Facultad de Medicina, Departamento de Biología Celular, Histología, Embriología y Genética. Ciudad de Buenos Aires, Argentina.Previously we showed that tectal EphA3 stimulates axon growth of nasal retinal ganglion cells (RGC) toward the caudal tectum preventing them from branching in the rostral tectum. In order to investigate the signaling mechanisms involved in the EphA3-mediated RGC axon growth, we evaluated the role of beta1 integrin in this process. For this purpose, we cultured chicken embryo nasal retinal explants on poly-L-lysine/laminin and exposed them to control conditions, to EphA3 ectodomain (aggregated EphA3-Fc), to a blocking anti-Beta1 integrin antibody and to EphA3-Fc plus the blocking anti-Beta1 integrin antibody. Axon density, axon length, growth cone morphologies, interstitial axonal filopodia density and length were evaluated after 3 and 6 hs of treatment at phase contrast microscopy and with immunocytochemistry against acetylated tubulin.The results showed that EphA3-Fc increases axon density and length, increasing the proportion of extending growth cones and decreasing the density of interstitial filopodia, whereas anti-Beta1 integrin decreases all parameters evaluated. Anti-Beta1 integrin prevents EphA3-mediated axon growth and growth cone extension. These results show that Beta1 integrin activity is necessary for control and EphA3-mediated RGC axon growth.Supported by UBACYT 0769.Key words: axon growth, Eph/ephrin, Beta1 integrin, retinal ganglion cells, retinotectal system, chick embryo, development.