A dual mechanism for notch1 early ventralizing activity

ENCINAS, PAULA INÉS; LÓPEZ, SILVIA L.; CASTRO COLABIANCHI, AITANA M.

Santiago de Chile

Workshop; EMBO Workshop: Bridging cell and tissue mechanics to fate specification in development; 2019

EMBO/ Universidad de Chile

A dual mechanism for notch1 early ventralizing activityAitana Manuela Castro Colabianchi, Paula Inés Encinas, Silvia Liliana López, et al.Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas,Instituto de Biología Celular y Neurociencias ´Prof. E. De Robertis´ (IBCN), Facultad de Medicina.Laboratorio de Embriología Molecular ´Prof. Dr. Andrés E. Carrasco´, Capital Federal, ArgentinaWe have found an asymmetric distribution of Notch1 in Xenopus and zebrafish embryos. Thisconstitutes the first evidence of a ventrally enriched protein along the early dorsal-ventralaxis.Notch1 has an important role in the establishment of this axis, since its attenuation results inthe downregulation of ventral center genes and in the upregulation of dorsal genes, like the wnt/β-catenin downstream gene chordin. The opposite happens when NICD1 is overexpressed. Inaddition, Notch1 contributes to the destabilization of β-catenin protein in the ventral side (Acostaet. al, 2011; Castro Colabianchi et. al, 2018 ). We propose that two different mechanisms coexistfor the notch ventralizing activity. Here we show a model for Notch1 and beta-catenin earlyinteraction.