Dynamics of cortico-angiogenesis in the developing chick optic tectum.

RODRIGUEZ CELIN, ALEJANDRA; RAPACIOLI, MELINA; DUARTE, SANTIAGO; ORTALLI, ANA L; TERUEL, LUISA R; SANCHEZ, VIVIANA; SCICOLONE, GABRIEL; LOPEZ, JUAN JOSÉ; FLORES, VLADIMIR

San Francisco, USA

Congreso; Society for Developmental Biology 68th Annual Meeting; 2009

Society for Developmental Biology

Society for Developmental Biology 68th Annual MeetingSan Francisco, USA 23-27 July, 2009Dynamics of cortico-angiogenesis in the developing chick optic tectumRodríguez Celín A, Rapacioli M, Duarte S, Ortalli A, Teruel L, Sánchez V,Scicolone G, López Costa JJ, Flores VThis work analyzes the temporal-spatial correlation between angio- and corticogenesis in thedeveloping chick optic tectum (OT) by means of NADPH-diaphorase and immunolabeling methods.Qualitative and quantitative parameters were used to analyze new vessels formation and growth ofpreexisting ones as a function of time and space. Angiogenesis begins with the radial ingression ofcapillary-like vessels, at the cephalic-basal mesencephalon, which by terminal anastomosis form asubventricular plexus (SVP). The tangential expansion of the SVP is accompanied by thesimultaneous ingression of additional vessels originated as radial branches from the external plexusoverlying the pial surface. As corticogenesis progresses by the addition of concentric neuronallayers, new vessels, with different spatial orientation, sprout from the previously formed radialvessels. A clear temporal correlation was observed between sprouting of new branches andformation of new neuronal layers. Besides, there is a remarkable spatial correlation between theincrease in complexity of the OT cortex and that of the vascular beds. At least five vascular bedswith different spatial patterns and specific positions respect to the successively appearing corticallayer were identified at the end of development. Angio- and corticogenesis progress as a function oftime and space from the cephalic to the caudal OT ends. Our results could advantageously help toanalyze the signaling networks involved in regulating the cortico-angiogenesis.Supported by grants from CONICET (Argentina)