Refinement of a femur surgery to test dental implants in rats: a pilot study

AGUSTINA RESASCO; CLARA VERCELLINI; MARTIN CARRIQUIRIBORDE; ANA CRISTINA CARRANZA MARTIN; FABRICIO MASCHI; MIGUEL ANGEL AYALA; MARÍA DEL PILAR CAGLIADA; KATYA KLUG; JOSE BELTRANO; JUAN LABORDE; CECILIA CARBONE

Mar del Plata

Congreso; Reunión Anual de Biociencia 2019; 2019

SAIC . SAFE . SAB . SAP . AACYTAL . NANOMED-ar . HCS

The refinement of the different techniques is an important part of Laboratory Animal Science. In our lab we have stablish a model to bone regeneration.In order to refine the technique, we aimed to study how two types of bone perforation (one long defect vs two smaller ones) would affect animal welfare. For this purpose, 8 WKAH/HokLAE rats were employed, housed in SPF conditions. After the surgical procedure, pain management and antibiotic therapy were given to the rats. Their weight, food consumption and performance in the burrowing test were measured before and after the surgery, and compared using paired t-tests. No significant differences in food consumption were detected between groups after a week (p≥0.05). Nevertheless, a trend in body weight reduction was observed after that period for the group with the long defect (p≥0.05≤0.1), whereas significant differences were observed for the rats with the double defect (p≤0.05). Regarding the burrowing test, significant differences were observed 72 hs after the surgery only in the rats with the long defect (p≤0.05). The original values for the burrowing test were restored when the it was repeated a week after the procedure (p≥0.05). As deviations from the original values were observed, further research into this two models will be performed in order to improve the refinement of this technique.