IBCN   20355
INSTITUTO DE BIOLOGIA CELULAR Y NEUROCIENCIA "PROFESOR EDUARDO DE ROBERTIS"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
TREM-1/TREM-2 ROLE IN REACTIVE ASTROGLIAL POLARIZATION TO THE PROINFLAMMATORY PHENOTYPE
Autor/es:
ROSCISZEWSKI, G; RAMOS AJ; CADENA V; CIERI B; VILLARREAL A
Lugar:
Córdoba
Reunión:
Congreso; XXXIII Reunión Anual de la Sociedad Argentina de Investigación en Neurociencias; 2018
Institución organizadora:
Sociedad Argentina de Neurociencias
Resumen:
Reactive gliosis is a generic astroglial response to brain injury. Reactive astrocytes can further polarize into an A1 proinflammatory-neurodegenerative phenotype. We have recently described that TLR4/NkB signaling facilitates astroglial conversion to the A1 phenotype (Rosciszewski et al., Mol. Neurobiol. 2017). Having in mind that TREM2/TREM1 and DAP12 participate in the fine-tuning of the inflammatory response by controlling TLR/NFkB signaling in immunocompetent cells, we here studied the expression of these receptors and DAP12 intracellular adaptor in vivo after brain ischemia and in vitro in glial cell cultures exposed to oxygen-glucose deprivation for 6 h. Using an experimental model of brain ischemia in rats, we detected TREM2 and DAP12 expression in glial cells, with a peak between 3-7 DPI with a specific localization in the ischemic penumbra. In vitro, we observed that OGD exposure increases TREM2 expression in astrocytes and microglia; reduces TREM1 in both cell types; while DAP12 expression is not significantly altered by OGD. Finally, we performed co-culture experiments of ischemic explants (3DPI) on primary glial cells. After 5 DIV, we observed that infiltrated cells from ischemic explants and mainly microglia expressed TREM2. Our results show that ischemia or OGD induces the expression of TREM1 and TREM2 in microglia but also in a subpopulation of reactive astrocytes and the DAP12 adaptor is available to signal in these cells. Grants: PICT 2015-1451; UBACYT.