Effect of CB1 receptor modulation on gene expresion in light induced retinal degeneration.

REY-FUNES, M.; PELAEZ R; GIRARDI E; SOLIÑO M.; LÓPEZ EM; MARTÍNEZ A; BAREIRO M.; LARRAYOZ I; LOPEZ JJ

Cordoba

Congreso; XXXIII Congreso Anual SAN 2018; 2018

Sociedad Argentina de Investigación en Neurociencias.

Light induced retinal degeneration (LIRD) is a model that resembles human retinal degenerative diseases as AMD. Endocannabinoids are neuromodulators whose effects are mediated by G protein coupled receptors named CB1 and CB2. Our previous results showed that the administration of ACEA (CB1 agonist) before continuous illumination stress is neuroprotective, decreasing apoptosis and glial reactivity, while AM251 (CB1 antagonist) worsened these parameters in LIRD. Our aim was to evaluate the effect of CB1 modulation on gene expression in LIRD. The right eyes of rats were intravitreally injected either with ACEA or AM251 while the left eyes received vehicle as controls. Later, rats were subjected to continuous illumination (12.000 lux) for 24 hs. Retinas were dissected and were processed by qRT-PCR. Data were statistically analysed using Student´s t-test and differences were considered significant when p