Cortico-angiogenesis dynamics in the developing chick optic tectum corticogenesis

RODRIGUEZ CELIN, ALEJANDRA; RAPACIOLI, MELINA; DUARTE, SANTIAGO; TERUEL, LUISA R; SCICOLONE, GABRIEL; LOPEZ-COSTA, JUAN JOSE; FLORES, VLADIMIR

Huerta Grande, Cordoba, Argentina

Congreso; First Joint Meeting of the Argentine Society for Neurosciences (SAN) and the Argentine Workshop in Neurosciences (TAN). IBRO/LARC.; 2009

Argentine Society for Neurosciences (SAN) and the Argentine Workshop in Neurosciences (TAN).

CORTICO-ANGIOGENESIS DYNAMICS IN THE DEVELOPING CHICK OPTIC TECTUM     Rodriguez Celin, A ; Rapacioli, M; Duarte, S ; Teruel,L; Scicolone G, Lopez-Costa J; Flores V.   This work analyzes the temporal-spatial correlation between angio- and corticogenesis in the developing chick optic tectum (OT) by means of the NADPH-diaphorase method. Qualitative and quantitative parameters were used to analyse new vessels formation and grow of preexisting ones a function of time and space. Angiogenesis begins with the radial ingression of capillary-like vessels, al the cephalic-basal mesencephalon, which by terminal anastomosis form a subventricular plexus (SVP). The tangential expansion of the SVP is accompanied by the simultaneous ingression of addicional vessels originated as radial branches from the external plexus overlyng the pial surface. As corticogenesis progresses by the addicio of concentric neuronal layers, new vessels, with different spatial orientation, sprout from the previously formed radial vessels. A clear temporal correlation was observed between sprouting of new branches and formation of new neuronal layers. Besides, there is a remarkable spatial correlation between the increase in complexity of the OT cortex and that of the vascular beds. At least four vascular beds with different spatial patterns and specific positions respect to the successively appearing cortical layer were identified at the end of development. Angio- and corticogenesis progress as a function of time and space from the cephalic to the caudal OT ends. Our results could advantageously help to analyze the signaling networks involved in regulating the cortico-angiogenesis. Supported by grants from CONICET (Argentina)