Adenosine A1 receptor agonist induces gene expression changes in light induced retinal degeneration

SOLIÑO M, LÓPEZ EM, REY-FUNES M, GIRARDI E Y LÓPEZ-COSTA JJ; LARRAYOZ I, MARTÍNEZ A

Washington

Congreso; Neuroscience 2017 - SfN?s 47th annual meeting; 2017

Society for Neuroscience

Adenosine A1 receptor agonist induces gene expression changes in light induced retinal degenerationSoliño M1, Larrayoz I2, López EM1, Rey-Funes M1, Martínez A2, Girardi E1 y López-Costa JJ1.A1 receptor (A1R) has been described in the retina and has been linked to the control of the characteristics of the subretinal space. A1R plays a role in inflammation, neuronal and trophic factor activity. Recently, in our lab, the modulation of A1R emerged as an effective neuroprotective strategy of the retina in the model of light induced retinal degeneration (LIRD). In order to further characterize the neuroprotective effects underlying A1R agonist treatment, mRNA changes induced by N6-cyclopentyladenosine (CPA), an A1R agonist, were studied in the retina using a LIRD model.Sprague Dawley rats received a single intravitreal injection of either CPA or vehicle, and then were subjected to continuous illumination (CI) (12000 lux) during 1 day. After this, the retinas were extracted and processed by RT-PCR. We selected as genes of interest adenosine receptors (A1, A2a, A2b and A3), proangiogenic factors (adrenomedullin and VEGF), an antiangiogenic factor (PEDF), inflammation mediators (IL-1b, iNOS, nNOS, and TNF) and a glial reactivity marker (GFAP). Data were statistically analysed using t-test on the Graphpad Prism software.RT-PCR results showed a significant decrease of A1, A2a, and A2b receptor mRNA in the retina of CPA treated eyes (p